AAV-CAG-GFP-httQ72 (Serotype 2)

Product Category: Pre-made recombinant AAV | AAV2-CAG-GFP-httQ72 | AAV-GFP-httQ72

Description:
AAV2-CAG-GFP-httQ72 is a pre-made recombinant adeno-associated virus (rAAV) packaged in serotype 2 (AAV2 capsid with AAV2 ITRs) designed for robust expression of a GFP-tagged huntingtin (HTT) protein fragment containing 72 CAG repeats (encoding glutamine, Q), representing a pathogenic polyglutamine (polyQ72) expansion associated with Huntington’s disease. Expression is driven by the strong, ubiquitous CAG promoter (CMV enhancer/chicken β-actin promoter), enabling high-level transgene expression across a wide range of mammalian cell types, including neurons.

The GFP fusion allows real-time visualization of mutant HTT protein localization, aggregation, and inclusion body formation in both in vitro and in vivo systems. The expanded polyQ72 tract promotes misfolding and aggregation, recapitulating key pathological features of Huntington’s disease, making this vector a powerful tool for studying disease mechanisms, neuronal toxicity, and protein aggregation dynamics.

This vector is ideal for neuroscience research, neurodegeneration studies, and disease modeling applications. Supplied in a ready-to-use format, AAV2-CAG-GFP-httQ72 provides reliable transduction efficiency and consistent expression in both cell culture and animal models.