AAV-CAG-GFP-httQ23 (Serotype 2)

Product Category: Pre-made recombinant AAV | AAV2-CAG-GFP-httQ23 | AAV-GFP-httQ23

Description:
AAV2-CAG-GFP-httQ23 is a pre-made recombinant adeno-associated virus (rAAV) packaged in serotype 2 (AAV2 capsid with AAV2 ITRs) designed for robust expression of a GFP-tagged huntingtin (HTT) protein fragment containing 23 CAG repeats (encoding glutamine, Q), representing a non-pathogenic polyglutamine (polyQ23) tract of the wild-type form. Expression is driven by the strong, ubiquitous CAG promoter (CMV enhancer/chicken β-actin promoter), enabling high-level transgene expression across a broad range of mammalian cell types, including neurons.

The GFP fusion enables direct visualization of HTT protein localization, aggregation dynamics, and intracellular trafficking in both in vitro and in vivo systems. As a non-pathogenic control, httQ23 serves as a critical baseline comparator for studies involving mutant huntingtin variants with expanded polyglutamine repeats in Huntington’s disease research.

This vector is well-suited for neuroscience, protein localization, and disease modeling applications, offering reliable transduction efficiency and consistent expression. Supplied in a ready-to-use format, AAV2-CAG-GFP-httQ23 is suitable for both cell culture and animal studies.