AAV-CAG-GCaMP6m (AAV Serotype 1)

Product Category: Pre-made recombinant AAV; AAV-CAG-GCaMP6m; AAV-CBA-GCaMP6m; AAV1-CAG-GCaMP6m; AAV1-CBA-GCaMP6m

Description:
GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI’s are that they can be genetically specified for studies in living organisms.

GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast – GCaMP6m, slow – GCaMP6m and medium – GCaMP6m).

The AAV1-CAG-GCaMP6m is a pre-packaged rAAV in serotype 1 (with capsid from AAV serotype 1 and 2xITR from AAV serotype 2) which over-expresses genetically encoded calcium indicator GCaMP6m under CAG promoter (also known as CBA promoter).

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

Vector Map: