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Off-the-shelf viral vectors for overexpression of firefly luciferase for in vivo imaging
Off-the-shelf viral vectors expressing firefly luciferase provide a convenient and reliable solution for bioluminescent in vivo imaging studies. These ready-to-use vectors are engineered for robust transgene expression, enabling sensitive, noninvasive monitoring of gene expression, cell viability, tumor progression, and therapeutic responses in living animals. Available in multiple formats—such as adenoviral (AdV), adeno-associated viral (AAV), and lentiviral (LV) systems—and under commonly used promoters (e.g., CMV, EF1α, or tissue-specific promoters), they support a wide range of experimental designs. Produced at high titers and quality-controlled for performance, these vectors allow rapid study initiation without the time and cost associated with custom vector construction, making them ideal for biodistribution, longitudinal tracking, and preclinical imaging applications.
Firefly Luciferase (fLuc)
The classical and most widely used bioluminescent reporter system, firefly luciferase (fLuc) utilizes the substrate D-luciferin and emits yellow-green light (~560 nm). At physiological temperature (37 °C), its emission spectrum shifts toward longer wavelengths, enhancing light penetration through deep tissues and improving detection sensitivity in vivo.
Explore our pre-made viral vectors for firefly luciferase (fLuc) overexpression, optimized for robust performance in in vivo imaging applications.
| SKU # | Viral Vector | Unit Size | Titer |
|---|---|---|---|
| SL100330 | LV-CMV-Luc-EF1a-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100709 | Ad-CMV-Luc | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL101432 | AAV8-CMV-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101434 | AAV1-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101440 | AAV9-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101493 | AAV1-CMV-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101494 | AAV9-CMV-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101495 | AAV1-CMV-Luc-PEST | 10~ 30 µL | >1E+13 VG/mL |
| SL101498 | AAV6-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101503 | AAV2-CMV-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101534 | AAV9-CMV-Rluc | 10~ 30 µL | >1E+13 VG/mL |
| SL101535 | AAV8-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101539 | AAV5-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL101735 | Ad-CMV-AkaLuc | 10~ 30 µL | >1E+13 VG/mL |
| SL101736 | Ad-CMV-Venus-T2A-AkaLuc | 10~ 30 µL | >1E+13 VG/mL |
| SL101737 | Ad-CMV-Cre-T2A-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116064 | AAV9-CMV-mCherry-T2A-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116067 | AAV9-CAG-Luc-T2A-GFP | 10~ 30 µL | >1E+13 VG/mL |
| SL116083 | AAV2-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116147 | AAV5-CMV-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116149 | AAV6-CMV-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116186 | AAV8-CAG-Luc-T2A-GFP | 10~ 30 µL | >1E+13 VG/mL |
| SL116259 | AAV(BI30)-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116261 | AAV2-CAG-Luc-T2A-GFP | 10~ 30 µL | >1E+13 VG/mL |
| SL116262 | AAV5-CAG-Luc-T2A-GFP | 10~ 30 µL | >1E+13 VG/mL |
| SL116335 | AAV(BI30)-CAG-Luc-T2A-GFP | 10~ 30 µL | >1E+13 VG/mL |
| SL116403 | AAV(rh74)-CAG-Luc | 10~ 30 µL | >1E+13 VG/mL |
| SL116465 | AAVhu68-CMV-fLuc | 10~ 30 µL | >1E+13 VG/mL |
| SL116484 | AAV9-CBh-AkaLuc-WPRE | 10~ 30 µL | >1E+13 VG/mL |
Interested in custom viral vectors for in vivo imaging?
SignaGen provides custom viral vector solutions for sensitive and reliable in vivo imaging. We design and produce AAV, lentiviral, and adenoviral vectors expressing fluorescent and bioluminescent reporters (e.g., GFP, mCherry, luciferase), optimized for tissue-specific expression and strong signal output. With high-titer production and rigorous QC, our vectors support real-time visualization of gene expression, cell tracking, and disease progression in diverse in vivo models.
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