Description:
GenMute™
siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting
siRNA to Hela cells. GenMute™ Reagent, 1.0
ml, sufficient for ~833 reactions based on transfecting 10 pmoles
siRNA or miRNA mimics in 24-well plate.
Refer to the following optimal transfection conditions for maximal silencing on
Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA
Complex
Maximal Silencing Efficiency
Transfection
Results:
Reporter Gene
Silencing
Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS
~50%
20.0 nM
1xGenMute™
Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
90% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
