Description
GenJet™ DNA In Vitro
Transfection Reagent for
epithelial cell is pre-optimized for epithelial
cells. By cross-linking a peptide ligand, this pre-optimized
GenJet™ reagent specifically
binds epithelial cells
and give superior efficiency on epithelial cells.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on epithelial cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to
1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours
EGFP
pEGFP-N3 (CMV promoter)
65% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A comparison showing superior efficiency of the
pre-optimized GenJet (Ver.
II) on normal human colonic epithelial
cells. Transfection was performed per the vendors' protocol. 0.5
µg
of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human
colonic epithelial cells with different transfection methods. The GFP
positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope
24 hours post transfection.
Data Sheet
