Description:
GenMute™
siRNA Transfection Reagent for HepG2 Cell is pre-optimized for transfecting
siRNA to HepG2 cells.
HepG2 (Hepatocellular carcinoma, human) is a perpetual add cell line which
was derived from the liver tissue of a 15 year old caucasian male with a well
differentiated hepatocellular carcinoma. These cells are epithelial in
morphology, have a model chromosome number of 55 and are not tumorigenic in nude
mice. The cells secrete a variety of major plasma proteins e.g.. albumin, alpha
2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They
have been grown successfully in large scale cultivation systems. Hepatitis
B virus surface antigens have not been detected. HepG2 cells have been shown to
be G418 resistant (400µg/mL). The cells will respond to stimulation with human
growth hormone.
Refer to the following optimal transfection conditions for maximal silencing on
HepG2 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA
Complex
Maximal Silencing Efficiency
Transfection
Results:
Reporter Gene
Silencing
Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS
~50%
40 nM
1xGenMute™
Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
85% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
