Description:
GenMute™
siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting
siRNA to C2C12 cells. C2C12
cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage
of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush
injury. These cells were shown to be capable of differentiation. C2C12 cells are
a useful model to study the differentiation of non-muscle cells to skeletal
muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins
and the androhen receptor (AR).
Size & content:
-
GenMute™ Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on C2C12 cells.
GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA
Complex
Maximal Silencing Efficiency
Transfection
Results:
Reporter Gene
Silencing
Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS
~50%
40 nM
1xGenMute™
Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
80% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
