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Cre & FLPo Recombinase Systems
SignaGen offers a comprehensive portfolio of pre-manufactured viral vectors designed for tightly regulated, inducible gene expression in mammalian systems. In parallel, we provide full-service custom vector engineering and production using adenoviral, adeno-associated viral (AAV), and lentiviral platforms to generate inducible expression constructs optimized for specific promoters, regulatory elements, and transgenes of interest. Our scientific support team delivers detailed vector maps, induction kinetics, and empirically guided dosing recommendations to suppress basal promoter activity and minimize off-target or background expression (“leakiness”). These measures enable high dynamic range, reproducible induction and ensure experimental fidelity in studies requiring precise temporal and quantitative control of gene expression.
Off-the-shelf viral vectors for inducible gene expression
Cre & FLPo Recombinase Systems
Cre, iCre, and Flpo are site-specific recombinase systems that act as precise “genetic scissors” to regulate gene expression with high spatial and temporal resolution. Cre and iCre recombinases recognize loxP sites, whereas Flpo—an optimized variant of the yeast-derived Flp recombinase—targets FRT sites. Upon expression, these enzymes mediate site-specific recombination events, resulting in deletion, inversion, or translocation of DNA segments flanked by their respective recognition sequences.
The combined use of the Cre/loxP and Flpo/FRT systems, an approach known as intersectional genetics, enables selective genetic manipulation of cell populations defined by the overlap of two independent markers (e.g., cells expressing both Gene A and Gene B). This dual-recombinase strategy is widely employed for generating conditional knockout models, neuronal circuit mapping and labeling, and dissecting complex developmental lineages, while minimizing off-target genetic modifications at the organismal level.
Refer to the table below for a detailed comparison of the features and applications of Cre, iCre, and Flpo recombinases.
| Feature | Cre | iCre (Improved Cre) | FLPo (Optimized FLP) |
| System | Cre-Lox | Cre-Lox | FLP-FRT |
| Origin | P1 Bacteriophage | P1 Bacteriophage (Modified) | Yeast (S. cerevisiae) |
| DNA Target | loxP sites | loxP sites | FRT sites |
| Optimization | None (Prokaryotic) | Mammalian Codon Optimized | Mammalian Codon Optimized |
| Efficiency | High | Very High | High (comparable to Cre) |
| Thermostability | Stable at 37°C | Stable at 37°C | Enhanced (Stable at 37°C) |
| Epigenetic Risk | High (CpG rich) | Low (Reduced CpG) | Low |
Explore our pre-made viral vectors based on Cre & FLPo recombinase systems, designed for inducible gene expression.
| SKU # | Viral Vector | Unit Size | Titer |
|---|---|---|---|
| SL100272 | LV-CMV-Cre-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100277 | LV-CMV-Cre-GFP-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100278 | LV-CMV-Cre-mCherry-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100279 | LV-EF1α-Cre-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100297 | LV-EF1α-iCre-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100280 | LV-EF1α-Cre-GFP-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100281 | LV-EF1α-Cre-mCherry-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100282 | LV-CAG-Cre | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100283 | LV-CAG-Cre-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100284 | LV-CAG-Cre-mCherry | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100294 | LV-Synapsin-Cre | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100295 | LV-Synapsin-Cre-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100296 | LV-CMV-iCre-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100298 | LV-Synapsin-iCre | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100299 | LV-EF1a-FLPo-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100300 | LV-CMV-FLPo-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100338 | LV-Syn-Cre-Syn-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100359 | LV-Syn-FLPo | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100360 | LV-CAG-FLPo | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100373 | LV-CAG-Cre-P2A-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100376 | LV-EF1a-Cre-T2A-Luc-IRES-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100377 | LV-Ef1a-iCre-P2A-GFP-Puro | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100380 | LV-Syn-iCre-P2A-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100383 | LV-GFAP-iCre-P2A-GFP | 25 ~ 50 µL | >1E+9 TU/mL |
| SL100706 | Ad-Cre-GFP | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL101086 | Ad-CMV-Cre-GFP (fusion) | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL101087 | Ad-CMV-Cre-CMV-mCherry | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL100707 | Ad-CMV-Cre | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL101173 | Ad-CMV-iCre | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL101174 | Ad-CMV-FLPo | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL101737 | Ad-CMV-Cre-T2A-fLuc | 50 ~ 200 µL | >1E+10 PFU/mL |
| SL100804 | AAV1-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100804-SC | scAAV1-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100805 | AAV1-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100881 | AAV1-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100882 | AAV1-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101117 | AAV1-CAG-Cre-mCherry | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101414 | AAV1-CAG-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101426 | AAV1-CMV-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101409 | AAV1-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101441 | AAV1-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101447 | AAV1-Syn-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101455 | AAV1-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101461 | AAV1-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101511 | AAV1-TRE-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116273 | AAV1-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100813 | AAV2-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100813-SC | scAAV2-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100814 | AAV2-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100884 | AAV2-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100891 | AAV2-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101410 | AAV2-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101415 | AAV2-CAG-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101427 | AAV2-CMV-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101442 | AAV2-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101448 | AAV2-Syn-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101456 | AAV2-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101462 | AAV2-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116274 | AAV2-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101283 | AAV3B-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101283-SC | scAAV3B-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101284 | AAV3B-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101289 | AAV4-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101289-SC | scAAV4-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101290 | AAV4-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100820 | AAV5-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100820-SC | scAAV5-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100821 | AAV5-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101400 | AAV5-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101435 | AAV5-CMV-iCe | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101443 | AAV5-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101449 | AAV5-Syn-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101453 | AAV5-CAG-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101457 | AAV5-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101505 | AAV5-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116275 | AAV5-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100827 | AAV6-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100827-SC | scAAV6-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100828 | AAV6-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101130 | AAV6-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101411 | AAV6-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101437 | AAV6-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101436 | AAV6-CMV-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101444 | AAV6-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101450 | AAV6-Syn-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101454 | AAV6-CAG-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101458 | AAV6-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116338 | AAV6-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116456 | AAV(Ark313)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116388 | AAV6.2-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116389 | AAV6.2-CMV-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100834 | AAV8-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100834-SC | scAAV8-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100835 | AAV8-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100887 | AAV8-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100883 | AAV8-Synn-Cre-Syn-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101429 | AAV8-ApoE/AAT1-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101412 | AAV8-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101416 | AAV8-CAG-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101438 | AAV8-CMV-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101445 | AAV8-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101451 | AAV8-Syn-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101459 | AAV8-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116277 | AAV8-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116376 | AAV8-TTR-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116485 | AAV8-Ef1a-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101276 | AAV(DJ/8)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101277 | scAAV(DJ/8)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101278 | AAV(DJ/8)-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101282 | AAV(DJ/8)-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101288 | AAV(DJ)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101292 | scAAV(DJ)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101293 | AAV(DJ)-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101297 | AAV(DJ)-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100841 | AAV9-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100841-SC | scAAV9-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100842 | AAV9-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101135 | AAV9-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100893 | AAV9-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100888 | AAV9-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL100892 | AAV9-Syn-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101431 | AAV9-αMHC-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101417 | AAV9-CAG-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101413 | AAV9-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101439 | AAV9-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101446 | AAV9-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101452 | AAV9-Syn-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101460 | AAV9-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101512 | AAV9-TRE-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116013 | AAV9-Syn-Cre-Syn-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116253 | AAV9-CAG-Cre-mCherry | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116278 | AAV9-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116486 | AAV9-EF1a-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101487 | AAV(PHP.B)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL101489 | AAV(PHP.B)-CMV-Cre-CMV-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116011 | AAV(PHP.eB)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116015 | AAV(PHP.eB)-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116068 | AAV(PHP.eB)-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116012 | AAV(PHP.eB)-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116340 | AAV(PHP.eB)-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116361 | AAV(PHP.eB)-CAG-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116378 | AAV(PHP.eB)-CMV-FLPo | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116385 | AAV(PHP.eB)-Syn-iCre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116399 | AAV(PHP.eB)-Syn-iCre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116453 | AAV(PHP.eB)-Syn-GFP-P2A-Cre (Serotype PHP.eB) | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116031 | AAV(PHP.S)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116032 | AAV(PHP.S)-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116034 | AAV(PHP.S)-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116235 | AAV(PHP.S)-CAG-Cre-mCherry | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116082 | AAV(2-Retro)-CMV-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116053 | AAV(2-Retro)-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116054 | AAV(2-Retro)-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116055 | AAV(2-Retro)-CAG-Cre-mCherry | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116058 | AAV(2-Retro)-Syn-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116059 | AAV(2-Retro)-Syn-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116060 | AAV(2-Retro)-Syn-Cre-mCherry | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116487 | AAV(2-Retro)-Ef1a-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116069 | AAV(BR1)-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116089 | AAV(BR1)-CAG-iCre-T2A-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116029 | AAV(BR1)-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116334 | AAV(BI30)-CAG-Cre | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116366 | AAV(BI30)-CAG-Cre-3xmiR122 | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116327 | AAV(BI30)-CAG-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116367 | AAV(BI30)-CAG-Cre-GFP-3xmiR122 | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116267 | AAV(BI30)-Cre-GFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116401 | AAV(BI30)-CAG-iCre-T2A-EGFP | 10 ~ 30 µL | >1E+13 VG/mL |
| SL116474 | AAV(BI30)-CAG-iCre-T2A-mCherry | 10 ~ 30 µL | >1E+13 VG/mL |
Interested in custom viral vectors for inducible gene expression?
Achieve precise control of gene expression with custom viral vectors designed for inducible systems such as Tet-On/Tet-Off. We provide end-to-end support—from construct design and plasmid preparation to viral packaging, purification, and quality control—ensuring tight regulation and robust induction. Optimized for both in vitro and in vivo use, our solutions deliver reliable and reproducible gene control.
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