Starting Materials to Run a ddPCR for rAAV Titration with An AutoDG Droplet Generator and QX 200 Droplet Reader

  • Molecular Biology Grade Water, Hyclone, SH30538.02
  • GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129
  • ddPCR Supermix for Probes no dUTP, Bio-Rad,1863023
  • 10% Pluronic F-68, Thermo Fisher, 24040032
  • Droplet generation oil, Bio-Rad, 1863005
  • DG32 cartridge, Bio-Rad, 1864108
  • ddPCR 96-well PCR plates, Bio-Rad, 12001925
  • Pierceable Foil Heat Seal, Bio-Rad, 1814040
  • Polystyrene Reservoirs, VWR, 89094-662
  • Microcentrifuge tubes, VWR, 87003-294
  • Primers/probe targeting ITR:
    • ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA
    • ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT
    • ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ-

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