qPCR Determination of Mycoplasma in AAV Samples

Generate primers are designed to amplify the mycoplasma 16S rDNA via qPCR.

Primer sequence:
16S U1 (forward) 5’–GTTTGATCCTGGCTCAGGAYDAAC– 3’ **
16S U8 (reverse) 5’–GAAAGGAGGTRWTCCAYCCSCAC– 3’ ***

Y = C or T; D = T, A or G; R = A or G; W = A or T; S = C or G.
** or 0.17 μM of each individual sequence assuming an equal proportion of concatenated nucleotide species at the degenerate position during oligonucleotide synthesis
*** that is 0.125 μM of each individual sequence