Plasmids for Optogenetics Sensors from Addgene

Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics sensors have been organized below into plasmids containing calcium sensors and other sensors.

Calcium Sensors

Reporter	Variant	Description	Plasmid(s)
GCaMP	GCaMP2	Modified calcium indicator derived from a fusion of a cpEGFP with C-terminus CaM and N-terminus M13 (GCaMP)	pCAGGS-GCaMP2 CMV-GCaMP2
	GCaMP3	Modified GCaMP	G-CaMP3
	GCaMP5G	Modified GCaMP	pCMV-GCaMP5G pN1 Lck-GCaMP5G
	GCaMP6s GCaMP6m GCaMP6f	Modified GCaMP	pGP-CMV-GCaMP6s GP-CMV-GCaMP6m pGP-CMV-GCaMP6f
GECO	G-GECO1.0	Genetically encoded calcium indicator (GECO)	CMV-G-GECO1.0 pTorPE-G-GECO1
	G-GECO1.1	Modified GECO	CMV-G-GECO1.1 pTorPE-G-GECO1.1
	G-GECO1.2	Modified GECO	CMV-G-GECO1.2 pTorPE-G-GECO1.2
	GEM-GECO1	Modified GECO	CMV-GEM-GECO1 CMV-mito-GEM-GECO1 pTorPE-GEM-GECO1
	R-GECO1.0	Modified GECO	CMV-R-GECO1 CMV-NLS-R-GECO pTorPE-R-GECO1
	GR-GECO1.1	Modified GECO	pCMV-GR-GECO1.1 pTorPE-GR-GECO1.1
	GR-GECO1.2	Modified GECO	pCMV-GR-GECO1.2 pTorPE-GR-GECO1.2
Twitch	Twitch-1	High-performing calcium indicator based on C-terminal domain of troponin C	Twitch-1 pRSETB
	Twitch-2		Twitch-2B pRSETB Twitch-2B pcDNA3
	Twitch-3		Twitch-3 pcDNA3 Twitch-3 pRSETB
	Twitch-4		Twitch-4 pcDNA3 Twitch-4 pRSETB
	Twitch-5		Twitch-5 pRSETB
TN-XXL	TN-XXL	TN-XXL	TN-XXL pRSETB TN-XXL pcDNA3

Other Sensors

Cellular Signal	Reporter	Variant	Description	Plasmid(s)
Chloride & pH	ClopHensor		Simultaneous, measurement of Cl^– and pH with E²GFP indicator	pcDNA3-ClopHensor pcDNA3-PalmPalm-ClopHensor
Chloride & pH	ClopHensorN		Simultaneous measurement of Cl- and pH optimized for use in the nervous system	ClopHensorN
Glutamate	iGluSnFR		Intensity-based glutamate-sensing fluorescent reporter (iGluSnFR)	pCMV(MinDis).iGluSnFR pRSET.GltI253-cpGFP.L1LV/L2NP
pH	sypHy		Fluorescent reporter of pH-sensitive pHluorin fused to synaptophysin	CMV::SypHy A4
Voltage	VSFP	VSFP2.1	Voltage-sensitive fluorescent protein (VSFP); Ci-VSP voltage sensor domain fused to a pair of fluorescent proteins (C-terminally truncated CFP and YFP)	pcDNA 3.1(-) VSFP2.1
		VSFP3.1	Modified VSFP with R217Q mutation in the S4 domain of Ci-VSP	pcDNA 3.1(-) VSFP3.1
		VSFP-CR	VSFP fused to Clover-mRuby2 FRET pair	pcDNA3.1/Puro-CAG-VSFP-CR
	ArcLight	ArcLight-S249	Modified ArcLight (voltage-sensing domain of Ci-VSP and super ecliptic pHluorin with A227D mutation) with pHluorin at position S249 in Ci-VSP	ArcLight-S249
		ArcLight-Q239	Modified ArcLight with pHluorin at position Q239 in Ci-VSP	ArcLight-Q239
		ArcLight-A242	Modified ArcLight with pHluorin at position A242 in Ci-VSP	ArcLight-A242
	ASAP	ASAP1	Accelerated Sensor of Action Potentials 1 (ASAP1), a cpGFP is inserted in an extracellular loop of a voltage-sensing domain	pcDNA3.1/Puro-CAG-ASAP1

One thought on “Plasmids for Optogenetics Sensors from Addgene”

Rotem Ben-Shushan says:
Dear Sir/Ms.
we are interesting at membrane potential detection in primary endothelial cell.
We want to get information about the arclight protein.

Regards,
Rotem, rotembs@migal.org.il