A single HEK293T cell can produce a wide range of adeno-associated virus (AAV) particles, typically in the range of 10,000 to 100,000 vector genomes per cell. The exact number varies depending on […]
Splice Donor and Acceptor Sites
Splice donor and acceptor sites are specific nucleotide sequences that define the boundaries between exons and introns during RNA splicing. Donor sites, located at the 5′ end of an intron (exon-intron boundary), […]
AAV Rh32.33 VP1 Gene
ATGGCTGCCGATGGTTATCTTCCAGATTGGCTCGAGGACAACCTCTCTGAGGGCATTCGCGAGTGGTGGGACCTGAAACCTGGAGCCCCCAAGCCCAAGGCCAACCAGCAGAAGCAGGACGACGGCCGGGGTCTGGTGCTTCCTGGCTACAAGTACCTCGGACCCTTCAACGGACTCGACAAGGGGGAGCCCGTCAACGCGGCGGACGCAGCGGCCCTCGAGCACGACAAGGCCTACGACCAGCAGCTCAAAGCGGGTGACAATCCGTACCTGCGGTATAACCACGCCGACGCCGAGTTTCAGGAGCGTCTGCAAGAAGATACGTCTTTTGGGGGCAACCTCGGGCGAGCAGTCTTCCAGGCCAAGAAGAGGGTACTCGAACCTCTGGGCCTGGTTGAAGAAGGTGCTAAGACGGCTCCTGGAAAGAAGAGACCGTTAGAGTCACCACAAGAGCCCGACTCCTCCTCAGGAATCGGCAAAAAAGGCAAACAACCAGCCAAAAAGAGACTCAACTTTGAAGAGGACACTGGAGCCGGAGACGGACCCCCTGAAGGATCAGATACCAGCGCCATGTCTTCAGACATTGAAATGCGTGCAGCACCGGGCGGAAATGCTGTCGATGCGGGACAAGGTTCCGATGGAGTGGGTAATGCCTCGGGTGATTGGCATTGCGATTCCACCTGGTCTGAGGGCAAGGTCACAACAACCTCGACCAGAACCTGGGTCTTGCCCACCTACAACAACCACTTGTACCTGCGGCTCGGAACAACATCAAACAGCAACACCTACAACGGATTCTCCACCCCCTGGGGATACTTTGACTTTAACAGATTCCACTGTCACTTCTCACCACGTGACTGGCAAAGACTCATCAACAACAACTGGGGACTACGACCAAAAGCCATGCGCGTTAAAATCTTCAATATCCAAGTTAAGGAGGTCACAACGTCGAACGGCGAGACTACGGTCGCTAATAACCTTACCAGCACGGTTCAGATATTTGCGGACTCGTCGTATGAGCTCCCGTACGTGATGGACGCTGGACAAGAGGGAAGTCTGCCTCCTTTCCCCAATGACGTCTTCATGGTGCCTCAATATGGCTACTGTGGCATTGTGACTGGCGAAAATCAGAACCAGACGGACAGAAATGCTTTCTACTGCCTGGAGTATTTTCCTTCACAAATGCTGAGAACTGGCAATAACTTTGAAATGGCTTACAACTTTGAGAAGGTGCCGTTCCACTCAATGTATGCTCACAGCCAGAGCCTGGACAGACTGATGAATCCCCTCCTGGACCAGTACCTGTGGCACTTACAGTCGACCACCTCTGGAGAGACTCTGAATCAAGGCAATGCAGCAACCACATTTGGAAAAATCAGGAGTGGAGACTTTGCCTTTTACAGAAAGAACTGGCTGCCTGGGCCTTGTGTTAAACAGCAGAGATTCTCAAAAACTGCCAGTCAAAATTACAAGATTCCTGCCAGCGGGGGCAACGCTCTGTTAAAGTATGACACCCACTATACCTTAAACAACCGCTGGAGCAACATAGCGCCTGGACCTCCAATGGCAACAGCTGGACCTTCAGATGGGGACTTCAGCAACGCCCAGCTCATCTTCCCTGGACCATCAGTCACCGGAAACACAACAACCTCAGCAAACAATCTGTTGTTTACATCAGAAGAAGAAATTGCTGCCACCAACCCAAGAGACACGGACATGTTTGGTCAGATTGCTGACAATAATCAGAATGCTACAACTGCTCCCATAACCGGCAACGTGACTGCTATGGGAGTGCTTCCTGGCATGGTGTGGCAAAACAGAGACATTTACTACCAAGGGCCAATTTGGGCCAAGATCCCACACGCGGACGGACATTTTCATCCTTCACCGCTAATTGGCGGTTTTGGACTGAAACATCCGCCTCCCCAGATATTTATCAAAAACACCCCCGTACCTGCCAATCCTGCGACAACCTTCACTGCAGCCAGAGTGGACTCTTTCATCACACAATACAGCACCGGCCAGGTCGCTGTTCAGATTGAATGGGAAATCGAAAAGGAACGCTCCAAACGCTGGAATCCTGAAGTGCAGTTTACTTCAAACTATGGGAACCAGTCTTCTATGTTGTGGGCTCCCGATACAACTGGGAAGTATACAGAGCCGCGGGTTATTGGCTCTCGTTATTTGACTAATCATTTGTAA
Deletion of the B-B’ or C-C’ in AAV ITR has a minimal impact on AAV production but increases transgene expression
1. ITR Structure and Function• ITRs are crucial: ITRs are key elements in AAV vectors, serving as origins of replication, and are essential for viral genome replication, packaging into capsids, […]
How Does pH Affect DNA Stability?
In a neutral pH range, pH 5 to 9, DNA molecules are quite stable. However, if the pH becomes too acidic or alkaline, DNA molecules are prone to destabilization. At […]
What is pH Value of ddH2O?
The pH of double-distilled water (ddH₂O) is theoretically around 7.0 — perfectly neutral — because pure water at 25°C autoionizes slightly into H₃O⁺ and OH⁻ ions. But in practice, ddH₂O […]