How to protect RNA from degradation?

To protect RNA from degradation, you can: 

• Inactivate RNases: Immediately after tissue harvesting, use a chaotropic-based cell lysis solution to homogenize samples and inactivate RNases. You can also flash freeze samples in liquid nitrogen. 
• Store at low temperatures: Store RNA at -70°C to -80°C for long-term storage, or at 4°C or -20°C for short-term storage. 
• Use RNase-free materials: Use RNase-free tubes, tips, water, buffers, and reagents. You can verify RNase-free status using RNase testing kits. 
• Add EDTA to chelate magnesium and other metals that can catalyze RNA degradation. Make sure to use an RNase-free EDTA solution. 
• Use RNAlater: Use RNAlater Tissue Collection Solution to stabilize and protect RNA in intact tissue and cell samples. 
• Wear gloves in the lab and change them often, especially after contact with skin, hair, doorknobs, keyboards, or animals. 

RNA is prone to enzymatic degradation and hydrolysis. Even at low temperatures, RNA can still be reactive, and some ribozymes can be enhanced by freezing.