Home made qPCR Master Mix

Reagent	Volume (for 150 µL)
qPCR mix (2×)	100 µL
Sense primer (1 µM)	2 µL
Antisense primer (1 µM)	2 µL
Fluorescein (1 µM)	2 µL
SYBR Green (40×) (Thermo Fisher Scientific S-7563)	2 µL
Taq DNA polymerase: JumpStart Taq Antibody (1:1) (New England Biolabs M0267X)	2 µL
Distilled water	40 µL
Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature.

qPCR Buffer (2×)

Reagent	Volume/final concentration (for 1 mL)
PCR buffer (10×; 200 mM Tris-HCl,  500 mM KCl, pH 8.4)	200 µL
MgCl2	25 mM
dNTPs	10 mM
Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature.