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Home made qPCR Master Mix
| Reagent | Volume (for 150 µL) |
|---|---|
| qPCR mix (2×) | 100 µL |
| Sense primer (1 µM) | 2 µL |
| Antisense primer (1 µM) | 2 µL |
| Fluorescein (1 µM) | 2 µL |
| SYBR Green (40×) (Thermo Fisher Scientific S-7563) | 2 µL |
| Taq DNA polymerase: JumpStart Taq Antibody (1:1) (New England Biolabs M0267X) | 2 µL |
| Distilled water | 40 µL |
| Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature. | |
qPCR Buffer (2×)
| Reagent | Volume/final concentration (for 1 mL) |
|---|---|
| PCR buffer (10×; 200 mM Tris-HCl, 500 mM KCl, pH 8.4) | 200 µL |
| MgCl2 | 25 mM |
| dNTPs | 10 mM |
| Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature. | |
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