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Good transgene expression measured by CAI
- Codon Adaptation Index (CAI) of your gene is 0.81. A CAI of 1.0 is considered ideal while a CAI of >0.8 is rated as good for expression in the desired expression organism. The lower the number, the higher the chance that your gene will be expressed poorly.
- The ideal percentage range of GC content is between 30% to 70%. Any peaks outside of this range will adversely affect transcriptional and translational efficiency.
- The percentage of low frequency (<30%) codons based on your target host organism is 1%. This un-optimized gene employs tandem rare codons that can reduce the efficiency of translation or even disengage the translational machinery.
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