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Commonly Used Primers
| 3’AOX1 | GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer |
| 5’AOX1 | GACTGGTTCCAATTGACAAGC For Pichia vectors with AOX1 promoter, forward primer |
| 35S promoter | CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer |
| AC5 | ACACAAAGCCGCTCCATCAG Drosophila Actin 5C promoter, forward primer |
| Alpha-factor | TACTATTGCCAGCATTGCTGC Alpha factor signal sequence, forward primer |
| Amp-R | ATAATACCGCGCCACATAGC 5′ end of ampicillin resistance gene, reverse primer |
| AUG1 Forward | CAATTTACATCTTTATTTATTAACG For Pichia vectors with AUG1 promoter, forward primer |
| AUG1 Reverse | GAAGAGAAAAACATTAGTTGGC For Pichia vectors with AUG1 promoter, reverse primer |
| BGH Reverse | TAGAAGGCACAGTCGAGG Bovine growth hormone terminator, reverse primer |
| Bglob-intron-F | CTGGTCATCATCCTGCCTTT Rabbit beta-globin intron, forward primer |
| Bglob-intron-R | TTTGCCCCCTCCATATAACA Rabbit beta-globin intron, reverse primer |
| Bglob-pA-R | TTTTGGCAGAGGGAAAAAGA Rabbit beta-globin polyA region, reverse primer |
| CAT-R | GCAACTGACTGAAATGCCTC 5′ end of chloramphenicol resistance gene, reverse primer |
| CMV Forward | CGCAAATGGGCGGTAGGCGTG Human CMV immediate early promoter, forward primer |
| CRE-R | GCAAACGGACAGAAGCATTT 5′ end of Cre recombinase, reverse primer |
| CYC1 | GCGTGAATGTAAGCGTGAC CYC1 transcription termination signal, reverse primer |
| DsRed1-C | AGCTGGACATCACCTCCCACAACG 3′ end of DsRed1, forward primer |
| DsRed1-N | GTACTGGAACTGGGGGGACAG 5′ end of DsRed1, reverse primer |
| EBV Reverse | GTGGTTTGTCCAAACTCATC SV40 polyA terminator, reverse primer |
| Ecdysone Forward | CTCTGAATACTTTCAACAAGTTAC Drosophila heat shock promoter, forward primer |
| EF-1a Forward | TCAAGCCTCAGACAGTGGTTC Human elongation factor-1a promoter, forward primer |
| EGFP-C | CATGGTCCTGCTGGAGTTCGTG 3′ end of EGFP, forward primer |
| EGFP-N | CGTCGCCGTCCAGCTCGACCAG 5′ end of EGFP, reverse primer |
| EXFP-R | GTCTTGTAGTTGCCGTCGTC For distinguishing EGFP vs ECFP vs EYFP, reverse primer |
| F1ori-F | GTGGACTCTTGTTCCAAACTGG F1 origin, forward primer |
| GAL1 | AATATACCTCTATACTTTAACGTC S. cerevisiae GAL1 promoter, forward primer |
| Gal10pro-F | GGTGGTAATGCCATGTAATATG S. cerevisiae GAL10 promoter, forward primer |
| Gal4 N-term | GAGTAGTAACAAAGGTCAA 3′ end of Gal4 DNA binding domain, forward primer |
| Gal4-AD | AATACCACTACAATGGAT 3′ end of Gal4 activation domain, forward primer |
| GFP-F | GGTCCTTCTTGAGTTTGTAAC 3′ end of GFP, forward primer |
| GFP-R | CCATCTAATTCAACAAGAATTGGGACAAC 5′ end of GFP, reverse primer |
| GPDpro-F | CGGTAGGTATTGATTGTAATTCTG S. cerevisiae GPD promoter, forward primer |
| GW-3′ | GCATGATGACCACCGATATG 3′ end of Gateway cassette, forward primer |
| GW-5′ | AATCTCGCCGGATCCTAACT 5′ end of Gateway cassette, reverse primer |
| H1 | TCGCTATGTGTTCTGGGAAA Human H1 promoter, forward primer |
| HA-F | TACCCATACGACGTCCCAGA HA tag, forward primer |
| HA-R | TCTGGGACGTCGTATGGGTA HA tag, reverse primer |
| HAT | GAGGAGCACGCTCATGCCCAC Histidine affinity tag, forward primer |
| hGH-PA-R | CCAGCTTGGTTCCCAATAGA Human growth hormone terminator, reverse primer |
| hrGFP-R | TCCCCGAGTACCACTTCATC hrGFP (humanized Renilla GFP), forward primer |
| hUBCpro-F | TGAAGCTCCGGTTTTGAACT Human Ubiquitin C (UbC) promoter, forward primer |
| IRES-F | TGGCTCTCCTCAAGCGTATT 3′ end of IRES, forward primer |
| IRES-R | CCTCACATTGCCAAAAGACG 5′ end of IRES, reverse primer |
| L4440 | AGCGAGTCAGTGAGCGAG 5′ of MCS in L4440 vector, forward primer |
| LacI-R | GGCATACTCTGCGACATCGT 5′ end of LacI, reverse primer |
| LacZ-R | GACAGTATCGGCCTCAGGAA 5′ end of LacZ, reverse primer |
| LexA | CGTCAGCAGAGCTTCACCATTG 3′ end of LexA DNA binding domain, forward primer |
| LKO.1 5′ | GACTATCATATGCTTACCGT Human U6 promoter, forward primer |
| LNCX | AGCTCGTTTAGTGAACCGTCAGATC (BD Biosciences) Human CMV promoter, forward primer |
| Luc-F | AGTCAAGTAACAACCGCGA 3′ end of luciferase, forward primer |
| LucNrev | CCTTATGCAGTTGCTCTCC 5′ end of luciferase, reverse primer |
| M13 (-21) Forward | TGTAAAACGACGGCCAGT In lacZ gene |
| M13 (-40) | GTTTTCCCAGTCACGAC In lacZ gene |
| M13 Reverse | CAGGAAACAGCTATGAC In lacZ gene |
| M13/pUC Forward | CCCAGTCACGACGTTGTAAAACG In lacZ gene |
| M13/pUC Reverse | AGCGGATAACAATTTCACACAGG In lacZ gene |
| MBP-F | GATGAAGCCCTGAAAGACGCGCAG 3′ end of maltose binding protein, forward primer |
| mCherry-F | CCCCGTAATGCAGAAGAAGA 3′ end of mCherry, forward primer |
| mCherry-R | TTGGTCACCTTCAGCTTGG 5′ end of mCherry, reverse primer |
| MT Forward | CATCTCAGTGCAACTAAA Drosophila metallothionein promoter, forward primer |
| MMLV-F | ATCAGTTCGCTTCTCGCTTC Moloney murine leukemia virus LTR (MoMuLV), forward primer |
| mPGK-F | CATTCTGCACGCTTCAAAAG Mouse PGK promoter, forward primer |
| MSCV | CCCTTGAACCTCCTCGTTCGACC Murine stem cell virus, forward primer |
| MSCV-rev | CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus, reverse primer |
| MT1-F | GCTGTCCTCTAAGCGTCACC Mouse metallothionein 1 promoter, forward primer |
| mU6-F | CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer |
| Myc | GCATCAATGCAGAAGCTGATCTCA (BD Biosciences) Myc tag, forward primer |
| Neo-F | CGTTGGCTACCCGTGATATT 3′ end of neomycin resistance gene, forward primer |
| Neo-R | GCCCAGTCATAGCCGAATAG 5′ end of neomycin resistance gene, reverse primer |
| NOS-F | GCGTTCAAAAGTCGCCTAAG Nopaline synthase promoter, forward primer |
| Nmt1-F | GCAATGTGCAGCGAAACTAA S. pombe nmt1 promoter, forward primer |
| OpIE2 Forward | CGCAACGATCTGGTAAACAC (Invitrogen) OpIE2 promoter, forward primer |
| pACYC-F | TGAAGTCAGCCCCATACGAT p15A origin, forward primer |
| pAd-CMV | GCTAGAGATCTGGTACCGTC For cloning sites after SalI in pAd-CMV vector |
| pBABE 3′ | ACCCTAACTGACACACATTCC (Weinberg Lab) SV40 enhancer, 3′ of MCS in pBABE vectors, reverse primer |
| pBABE 5′ | CTTTATCCAGCCCTCAC (Weinberg Lab) Psi packaging signal, 5′ of MCS in pBABE vectors, forward primer |
| pBAD Forward | ATGCCATAGCATTTTTATCC (Invitrogen) For vectors with E. coli araBAD promoter, forward primer |
| pBAD Reverse | GATTTAATCTGTATCAGG (Invitrogen) For vectors with E. coli araBAD promoter, reverse primer |
| pBluescriptKS | TCGAGGTCGACGGTATC For pBluescript vector |
| pBluescriptSK | TCTAGAACTAGTGGATC For pBluescript vector |
| pBMN 5′ | GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector |
| pBR322ori-F | GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer |
| pBRforBam | CTTGGAGCCACTATCGAC In pBR322 tet region, upstream of BamHI, forward primer |
| pBRforEco | AATAGGCGTATCACGAGGC In pBR322, upsteam of EcoRI site, forward primer |
| pBRrevBam | GGTGATGTCGGCGATATAGG In pBR322 tet region, downstream of BamHI, reverse primer |
| pCAG-F | GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids, forward primer |
| pCasper-F | GGGTTTTATTAACTTACAT 5′ end of Drosophila mini-white gene, reverse primer |
| pCasper-hs | GCAACTACTGAAATCTGCCAAG Drosophila Hsp70 promoter, forward primer |
| pcDL-F | GTTGCCTTTACTTCTAGGCCT 5′ of EcoRI site in pcDL vector, forward primer |
| pENTR-F | CTACAAACTCTTCCTGTTAGTTAG 5′ of attL1 in pENTR vector, forward primer |
| pENTR-R | ATGGCTCATAACACCCCTTG 3′ of attL2 in pENTR vector, reverse primer |
| pGEX 3′ | CCGGGAGCTGCATGTGTCAGAGG 3′ of MCS in pGEX vectors, reverse primer |
| pGEX 5′ | GGGCTGGCAAGCCACGTTTGGTG 3′ end of glutathione-S-transferase, forward primer |
| pGP704-R | AACAAGCCAGGGATGTAACG R6K gamma origin, 3′ of MCS in pGP704 vector, reverse primer |
| pHybLex Reverse | GAGTCACTTTAAAATTTGTATACAC ADH terminator, reverse primer |
| pLTet-F | ACTGAGCACATCAGCAGGAC Lambda phage early leftward (pL) promoter, forward primer |
| pLXSN 5′ | CCCTTGAACCTCCTCGTTCGACC (MSCV) Murine stem cell virus, same as MSCV, forward primer |
| pMRB101-F | AAGATGCAGGCAGCTGAGTT HCMV major immediate-early protein (IE), forward primer |
| pMT2-F | TTGCCTTTCTCTCCACAGGT 3′ end of synthetic intron, forward primer |
| pMX-S1811 | GACGGCATCGCAGCTTGGATACAC MMLV sequence, 5′ of MCS in pMXs vector, forward primer |
| Polyhedrin forward | AAATGATAACCATCTCGC Polyhedrin promoter, forward primer |
| Polyhedrin reverse | GTCCAAGTTTCCCTG For baculovirus vector with polyhedrin promoter, reverse primer |
| pQE promoter | CCCGAAAAGTGCCACCTG 5′ of MCS in pQE vectors, forward primer |
| pREP Forward | GCTCGATACAATAAACGCC Rous sarcoma virus (RSV) promoter, forward primer |
| pRS-marker | CGGCATCAGAGCAGATTGTA To sequence yeast selectable marker in pRS vectors |
| Pry1 | CTTAGCATGTCCGTGGGGTTTGAAT PZ P-element, reverse primer |
| pTrcHis Forward | GAGGTATATATTAATGTATCG 5′ of MCS in pTrcHis vector, forward primer |
| pTrcHis Reverse | GATTTAATCTGTATCAGG 3′ of MCS in pTrcHis vector, same as pBAD-R, reverse primer |
| Puro-F | GCAACCTCCCCTTCTACGAGC 3′ end of puromycin resistance gene, forward primer |
| pZIP | TCCTTTCCAGCGAGGTTCTA Murine leukemia virus (MuLV), reverse primer |
| RCAS-F | ACATGGGTGGTGGTATAGCGCTTGCG 3′ of Rous sarcoma virus (RSV) env gene, forward primer |
| Rluc-F | CCAGGATTCTTTTCCAATGC 3′ end of Renilla luciferase, forward primer |
| RVprimer3 | CTAGCAAAATAGGCTGTCCC 5′ of MCS in pGL3 vector, forward primer |
| SFFV-F | ATTGATTGACTGCCCACCTC Spleen focus forming virus 5′ LTR, forward primer |
| SP6 | ATTTAGGTGACACTATAG SP6 promoter, forward primer |
| SV40pA-R | GAAATTTGTGATGCTATTGC SV40 polyA, reverse primer |
| SV40pro-F | TATTTATGCAGAGGCCGAGG SV40 promoter/origin, forward primer |
| SV40-spliceR | CACAAAGATCCGGACCAAAG SV40 splice sequence, reverse primer |
| T3 | GCAATTAACCCTCACTAAAGG T3 promoter, forward primer |
| T7 | TAATACGACTCACTATAGGG T7 promoter, forward primer |
| T7 Terminal | GCTAGTTATTGCTCAGCGG T7 terminator, reverse primer |
| Tac promoter | GAGCGGATAACAATTTCACACAGG Tac promoter, forward primer |
| tdTomato-Fwd | CTGTTCCTGTACGGCATGG 3′ end of tdTomato, forward primer |
| tdTomato-Rev | TCTTTGATGACGGCCATGT 5′ end of tdTomato, reverse primer |
| Tet-R | GGCGAGTTTACGGGTTGTTA 5′ end of tetracycline resistance gene, reverse primer |
| TK-pA-R | TTGTCTCCTTCCGTGTTTCA Thymidine kinase polyA, reverse primer |
| Tn7-end | GGGGTGGAAATGGAGTTTTT Bacterial transposon Tn7 |
| TRC-F | CAAGGCTGTTAGAGAGATAATTGGA Human U6 promoter, forward primer |
| Ubx-F | AACTCGTACTTTGAACAGGC Drosophila Ultrabithorax gene, forward primer |
| V5 Reverse | ACCGAGGAGAGGGTTAGGGAT V5 epitope, reverse primer |
| WPRE-R | CATAGCGTAAAAGGAGCAACA 5′ end of WPRE, reverse primer |
| XBG-R | GACTCCATTCGGGTGTTC Xenopus beta-globin 3’UTR, reverse primer |
| XEF1a | TTTCGCCCTAACTTCGTGAT Xenopus EF1 alpha enhancer/promoter, forward primer |
| Xpress Forward | TATGGCTAGCATGACTGGT Xpress epitope, forward primer |
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