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Cell Culture Antibiotic Selection Guide
| Product Name | Gram (+)bacteria | Gram (-)bacteria | Yeasts | Molds | Mycoplasma | Suggested Working Conc. |
| Amphotericin B | † | † | 2.5 mg/L | |||
| Amphotericin B-Solubilized (Approx. 45%) | † | † | 5.6 mg/L (of solid) | |||
| Amphotericin B(250 μg/ml solution) | † | † | 10 ml/L | |||
| Ampicillin | † | † | 100 mg/L | |||
| Antibiotic Antimycotic Solution (100x)(10,000 units penicillin, 10 mg streptomycin, and 25 µg amphotericin B per ml) | † | † | † | † | 10 ml/L | |
| Cephalothin | † | † | 100 mg/L | |||
| Dihydrostreptomycin | † | † | 100 mg/L | |||
| Erythromycin | † | † | 100 mg/L | |||
| Gentamicin Sulfate | † | † | † | 50 mg/L | ||
| Gentamicin Sulfate (10 mg/ml solution) | † | † | † | 5 ml/L | ||
| Gentamicin Sulfate (50 mg/ml solution) | † | † | † | 1 ml/L | ||
| L-Glutamine-Penicillin-Streptomycin Solution (200 mM L- glutamine, 10,000 units penicillin, and 10 mg streptomycinper ml) | † | † | 10 ml/L | |||
| Kanamycin Monosulfate | † | † | † | 100 ml/L | ||
| Kanamycin Sulfate (10 mg/ml solution) | † | † | † | 10 ml/L | ||
| Kanamycin Sulfate (50 mg/ml solution) | † | † | † | 2 ml/L | ||
| Lincomycin HCI | † | 100 mg/L | ||||
| Neomycin Sulfate | † | † | 50 mg/L | |||
| Neomycin Sulfate (10 mg/ml solution) | † | † | 5 ml/L | |||
| Nystatin (5,000 units nystatin per mg) | † | † | 2.5 X 105U/L (50 mg/L) | |||
| Nystatin | † | † | 50 mg/L | |||
| Nystatin Suspension | † | † | 24 ml/L | |||
| Paromomycin Sulfate | † | † | 100mg mg/L | |||
| Penicillin-G (potassium salt) | † | 100,00 U/L | ||||
| Penicillin-G (sodium salt) | † | 100,000 U/L | ||||
| Penicillin-Streptomycin solution (5,000 units penicillin-G and 5 mg streptomycin per ml) | † | † | 20 ml/L | |||
| Penicillin-Streptomycin Solution (10,000 units penicillin-G and 10 mg streptomycin per ml) | † | † | 10 mg/L | |||
| Penicillin-Streptomycin-Neomycin solution (5,000 units penicillin-G,5 mg streptomycin and 10 mg neomycin per ml) | † | † | 10 ml/L | |||
| Phenoxymethylpenicillinic Acid (potassium salt) [Penicillin V] | † | 100,000 U/L | ||||
| Polymyxin B Sulfate | † | 50 mg/L | ||||
| SpectinomycinDihydrochloride | † | † | 7.5-20 mg/L | |||
| Spectromycin Sulfate | † | † | 10 mg/L | |||
| TetracyclineHydrochloride | † | † | 10 mg/L | |||
| Tylosin Tartrate(8 mg/ml soln.) | † | † | 1 ml/L | |||
| ANTIBIOTICS FOR PLANT CELL CULTURE | ||||||
| Product Name | Gram (+)bacteria | Gram (-)bacteria | Mycobacteria | Yeasts | Molds | Mycoplasma |
| Carbenicillin | ‡ | † | ||||
| Cefotaxime | ‡ | † | ||||
| Chloramphenicol | † | † | ‡ | ‡ | ||
| G 418 | ||||||
| Gentamicin Sulfate | ‡ | † | † | |||
| Hygromycin B | ||||||
| Kanamycin | † | † | † | |||
| Paromycin | † | ‡ | ||||
| Penicillin G, Potassium | † | ‡ | ||||
| Penicillin G, Sodium | † | ‡ | ||||
| Rifampicin | † | † | † | |||
| Streptomycin Sulfate | ‡ | † | ‡ | |||
| Vancomycin | † | |||||
| Product Name | Gram (+)bacteria | Gram (-)bacteria | Myco-bacteria | Yeasts | Molds | Myco-plasma |
| Nystatin | † | † | ||||
| Pentachloronitrobenzene | † | |||||
| Thiabendazole | † | |||||
| † = Effective against most species | ||||||
| ‡ = Effective against certain species | ||||||
| 1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant | ||||||
| selection; SE = somatic embryogenesis; SSC = stem selection culture; PC = protoplast culture; TEC = tuber explant culture; | ||||||
| STM = shoot tip micropropagation | ||||||
| ANTIBIOTIC SELECTION AGENTS FOR CELL CULTURE | ||||||
| Product Name | Mode of Action | Suggested Working Conc. | ||||
| Actinomycin D | Complexes with DNA and interferes with RNA synthesis | 1 µg/ml | ||||
| Bleomycin Sulfate | Complexes with DNA, causing strand scissions | 10-100 µg/ml | ||||
| Chloramphenicol | Inhibits elongation at peptidyl transferase | 5 µg/ml | ||||
| G 418 | Blocks polypeptide synthesis and inhibits chain elongation | 100-800 µg/ml | ||||
| G 418 (50 mg/ml solution) | Blocks polypeptide synthesis and inhibits chain elongation | 1 µg/ml | ||||
| Hygromycin B | Blocks polypeptide synthesis and inhibits chain elongation | |||||
| Mitomycin C | Inhibits nucleic acid synthesis | 10-50 µg/ml | ||||
| Mycophenolic Acid | Blocks inosine monophosphate dehydrogenase in guanosine monophosphate pathway | 25 µg/ml | ||||
| Puromycin HCl | Inhibits protein synthesis | 10-100 µg/ml |
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