CCAGAAATGGCGCCGGAGGCGGGAACAAGGTGGTGGATGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGC TCCAGTGGGCGTGGACTAATATGGAACAGTATTTAAG
Adeno-associated Virus (AAV) - 5. page
p40 Promoter
GGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAGTTCTACGTCAAAAAGGGTGGAGCCAAGAAAAG ACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCT
TATAless p5 Promoter
GGAGGGGTGGAGTCGTGACGTGAATTACGTCATAGGGTTAGGGAGGTCCTGTATTAGAGGTCACGTGAGTGTTTTGCGACATTTTGCGACACCA TGTGGTCTCGCTGGGGGGGGGGGCCCGAGTGAGCACGCAGGGTCTCCATTTTGAAGCGGGAGGTTTGAACT
qPCR Primer and Probe Sequences for rAAV Titration
CMV Promoter: CMV-F TTC CTA CTT GGC AGT ACA TCT ACG CMV-R GTC AAT GGG GTG GAG ACT TGG CMV-P TGA GTC AAA CCG CTA TCC ACG CCC A AAV2 […]
Wt AAV P5 Promoter for Rep
ggaggggtggagtcgtgacgtgaattacgtcatagggttagggaggtcctgtattagaggtcacgtgagtgttttgcgacattttgcgacaccatgtggtcacgctgggtatttaagcccg agtgagcacgcagggtctccattttgaagcgggaggtttgaac
rcAAV Assay via PCR
PCR and sequencing. The following primers were used for PCR amplification for analysis of rcAAV: D region, 5′-CTCCATCACTAGGGGTTCC-3′ (AAV2 nt 126 to 144); rep, 5′-GGCAGATGCCCGTCAAGGT-3′ (AAV2 nt 379 to 361); and cap, 5′-CAGAGATGTGTACCTTCAG-3′ […]