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Adeno-associated Virus (AAV) - 5. page

p19 Promoter

CCAGAAATGGCGCCGGAGGCGGGAACAAGGTGGTGGATGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGC TCCAGTGGGCGTGGACTAATATGGAACAGTATTTAAG

p40 Promoter

GGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAGTTCTACGTCAAAAAGGGTGGAGCCAAGAAAAG ACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCT

TATAless p5 Promoter

GGAGGGGTGGAGTCGTGACGTGAATTACGTCATAGGGTTAGGGAGGTCCTGTATTAGAGGTCACGTGAGTGTTTTGCGACATTTTGCGACACCA TGTGGTCTCGCTGGGGGGGGGGGCCCGAGTGAGCACGCAGGGTCTCCATTTTGAAGCGGGAGGTTTGAACT

Wt AAV P5 Promoter for Rep

ggaggggtggagtcgtgacgtgaattacgtcatagggttagggaggtcctgtattagaggtcacgtgagtgttttgcgacattttgcgacaccatgtggtcacgctgggtatttaagcccg agtgagcacgcagggtctccattttgaagcgggaggtttgaac

rcAAV Assay via PCR

PCR and sequencing. The following primers were used for PCR amplification for analysis of rcAAV: D region, 5′-CTCCATCACTAGGGGTTCC-3′ (AAV2 nt 126 to 144); rep, 5′-GGCAGATGCCCGTCAAGGT-3′ (AAV2 nt 379 to 361); and cap, 5′-CAGAGATGTGTACCTTCAG-3′ […]