Stable HT-29 clones with > 97% of all cells homogeneously expressing GFP were generated with the EF-1 alpha promoter. In contrast in clones carrying the CMV promoter, only up to […]
Lentivirus - 3. page
Why EF1alpha promoter is so special?
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. […]
Lentivirus Production Protocol
Supplies: 293FT Cells (Invitrogen: R700-07) T-225 tissue culture flasks (Nunc: 159934) T-75 tissue culture flasks (Nunc: 156499) 500 cm2 tissue culture plates (Nunc: 166508) Ultracentrifuge tubes (Beckman Culture: 344058) DMEM […]
Plasmids for Optogenetics Sensors from Addgene
Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics […]
Key properties of a viral vector
Viral vectors are tailored to their specific applications but generally share a few key properties: Safety: Although viral vectors are occasionally created from pathogenic viruses, they are modified in such […]
Pre-made Lentivirus from GeneCopoeia
Positive and negative controls: eGFP eYFP eCFP mCherry PLUM Firefly luciferase Renilla luciferase Gaussia luciferase Firefly luciferase + eGFP/mCherry Renilla luciferase + eGFP/mCherry Firefly luciferase + IRES-mCherry Renilla luciferase + […]
mRFP expression in neurones and glia in dorsal vagal and hypoglossal motor nuclei
Representative images are shown for the experimental conditions evaluated. mRFP-expressing cells transduced with either AVV- or LVV-CMV-mRFP are shown in the left hand panels; IR for NeuN or GFAP in […]
WHAT ARE SOME DIFFERENT WAYS TO TITER LENTIVIRUS?
Functional titer is defined as the number of functional vector particles required to infect a cell, present in a volume. There are several methods for measuring titer. Some are more […]
PRACTICAL APPLICATIONS AND LIMITATIONS OF LENTIVIRAL VECTOR
Applications of lentivirus vectors include stable gene transfer for gene delivery in gene and cell therapy. This includes using RNA interference technology to block the expression of specific genes. Along […]