Forward: 5′-AGCTTGCCTTGAGTGCTTCA-3′ Reverse: 5′-TGACTAAAAGGGTCTGAGGGA-3′
Lentivirus - 2. page
Optimal MOIs for Some Cells Infected with Lentivirus
Cell line Tissue Cancer/cell type Species MOI A431 Epithelial Carcinoma Human 5 A549 Lung Carcinoma Human 5 Astrocytes Nervous system Primary Human 1 B16-F10 Epithelial Melanoma, metastatic Mouse 5 BMM Bone Marrow Primary Human 8 […]
Going viral: chimeric antigen receptor T-cell therapy for hematological malignancies.
On July 1, 2014, the United States Food and Drug Administration granted ‘breakthrough therapy’ designation to CTL019, the anti-CD19 chimeric antigen receptor T-cell therapy developed at the University of Pennsylvania. […]
The CAR T-Cell Race
Tumor-targeting T-cell therapies are generating remarkable remissions in hard-to-beat cancers—and attracting millions of dollars of investment along the way. By Vicki Brower | April 1, 2015 BUILDING A BETTER T-CELL: […]
CAR T-CELL BIOTECH IPOs
Company Date Value Kite Pharma June 2014 $134.1 million Bellicum December 2014 $160 million Juno December 2014 $264.6 million Cellectis March 2015 $228 million
CAR T-CELL DEALS
Institution/Company Date Partner Terms University of Pennsylvania August 2012 Novartis Undisclosed Celgene March 2013 Bluebird Bio, Baylor College of Medicine Unspecified upfront payment plus up to $225 million per product […]
CAR T-Cell Therapy: Engineering Patients’ Immune Cells to Treat Their Cancers
For years, the cornerstones of cancer treatment have been surgery, chemotherapy, and radiation therapy. Over the last decade, targeted therapies like imatinib (Gleevec®) and trastuzumab (Herceptin®)—drugs that target cancer cells […]
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]
Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG […]