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Month September 2020
Bmi-1 Validated target Regions
GAGATAATAAGCTTGTCTA ACAACCAGAATCAAGATCA ATATGGATGTTAAGTGGAA
Validated siRNA Targeting Mouse Trpv1 Gene
5’-GTCGGTTTATGTTCGTCTAC-3’
A Novel AAV Serotype B1 for CNS Transduction
VP1 Amino Acid Sequence of AAV-B1: MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHAD VEFQERLQEDTSFGGNLGRAVFQAKKRVLEPFGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVG PNTMAAGGGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRD WQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYF PSQMLRTGNNFEFSYQFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTARTQQLLFSQAGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNN SNSAWTAGTKYHLNGRNSLANPGIAMATHKDDEERFFPSNGILIFGKQNAARDNADYSDVMLTSEEEIKTTNPVATEEYGIVADNLQQQNTAPQIGTVNSQ GALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEI QYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL
Novel AAV-F AAV-S for Neuronal Cortex Transduction
AAV-F and AAV-S were confirmed to mediate transgene expression in the brain cortex more…
Virus used in gene therapies may pose cancer risk, dog study hints
Just as gene therapy finally seems to be living up to its promise, a…
Why do I see amplification curves in my NTC samples?
Products can appear in non-template control (NTC) wells due to the ability of some…
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
There is DNA contamination somewhere in your PCR assay system. Use only PCR-grade reagents…
qPCR: Avoiding Signals in the No-template Control (NTC)
This month’s question from the Molecular Biology Forums (online at molecularbiology.forums.biotechniques.com) comes from the…
Human HEY1 siRNA Validated
5′-UAGAGCCGAACUCAAGUUUCCAUUC-3′ 5′-UUGAGAUGCGAAACCAGUCGAACUC-3′
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