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Year 2020
Validated Target Region for TLR4 Gene
5′- CCGTTGGTGTATCTTTGAATA -3′
Mouse EGR1 Validated Target Region
5′-GGACTTAAAGGCTCTTAAT-3′5′-GGACAAGAAAGCAGACAAA-3′
Human ChAT Promoter
cccgggagcagggggtggggaaggaggagcagtggctggtggcaagtctgagccaggacatagccgatgacagcctgtgaatgaccggccagactggggttggaccttcggggcagagcaggcagcccttggaaagccaggctggtaggagcatgggtgggaggcacaaaacacagacaaacagacatatatacacatatatcctctaggcatctccgaggagtgatggaggagagagttggtaggtgggaccacaggaaaccatcccacccagctctgccttcttcaagctcagggtccctgaagcctgactagtgtctcccgaatgggggaggggggggctctgggcagagcaaaaggaggcattggctgcatgtcttagggcctttaccaactgaccttgtccttgctgtcctctcaaaagactgccatgctccggtgaatcctcaccaggacgccacctctttcactttgcagttgggggcagcccctacgaacccacagcactgggaggcgtgggaacaagcctgtggagagtgtccctgcactgtgccagcaagggctttacattctctgcccagcactgcaaggcagccccaggctcctccatccttgggcctggttcatccatctttctggactctctgttcctggcaacaaccctgccagtcccgatacatgggcggtgcttgcctctgggtaggggtggtgttgggtggggagaggggtgaggaaattagctaaacctcagctttagacttgagagaatagagggtgggctggtggaaaacatgagtatgtgcctgtggaggcttaggttcagggggatctcagagggtagcaattgtgacccacagcctaataataacagtctttgccctcttggcctggctc
Validated Target Region against Mouse G3BP Gene
CCTCAGAGAGATCAGAGAGTT
Two Validated Mouse SV2C Target Regions
5′- GACAGCATCGTGTCTGTAG -3′5′- ATCGTGTCTGTAGGACAGC -3′
Validated Target Region against Mouse Cacna1c Gene
5′- GGAAAGCTCTATACCTGTT – 3′
Luna Universal Probe One-Step RT-qPCR for Detection of Covid-19
Reaction Setup: For best results, we recommend running each RNA standard and sample in triplicate.…
RT-qPCR Reaction Conditions for Detection of Covid-19 by FDA and CDC
In Stage 1, Set to 2 min at 25°C; 1 Rep.In Stage 2, Set…
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