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qPCR Primer and Probe Sequences for rAAV Titration

CMV Promoter:
CMV-F TTC CTA CTT GGC AGT ACA TCT ACG
CMV-R GTC AAT GGG GTG GAG ACT TGG
CMV-P TGA GTC AAA CCG CTA TCC ACG CCC A

AAV2 ITR:
ITR-F GGA ACC CCT AGT GAT GGA GTT
ITR-R CGG CCT CAG TGA GCG A
ITR-P CAC TCC CTC TCT GCG CGC TCG

Humanized Renilla Green Fluorescent Protein (hrGFP):
hrGFP-F GAT CCG CAG CGA CAT CAA CC
hrGFP-R GTA CAC CAC CTC GAA GCT GG
hrGFP-P GAG GAG ATG TTC GTG TAC CGC GTG G

SV40 Polyadenylation Signal:
SV40-F AGC AAT AGC ATC ACA AAT TTC ACA A
SV40-R CCA GAC ATG ATA AGA TAC ATT GA
SV40-P AGC ATT TTT TTC ACT GCA TTC TAGTTG TGG TTT GTC

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