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Month October 2019
qPCR Primer and Probe Sequences for rAAV Titration
CMV Promoter: CMV-F TTC CTA CTT GGC AGT ACA TCT ACG CMV-R GTC AAT…
Commonly Used Primers
3’AOX1 GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer 5’AOX1 GACTGGTTCCAATTGACAAGC For Pichia…
Sim1 Specific Promoter for PVN Neurons
1 GGTACCGAGG CGCGCAGCCG TGGGGAAATC CTGGCCACTG TCTGGGATGC 51 TATTCGCACG CGTTCCTGGA GCCTCGGCTA GCCAGCCCCC GCAAGGCCGC 101 ACGCTGTGCG…
Wt AAV2 p5 Promoter for Rep
ggaggggtggagtcgtgacgtgaattacgtcatagggttagggaggtcctgtattagaggtcacgtgagtgttttgcgacattttgcgacaccatgtggtcacgctgggtatttaagcccg agtgagcacgcagggtctccattttgaagcgggaggtttgaac
Validated Target Region against Mouse Slc16a7 (MCT2) Gene
CATTGGAGGTTTAGGATTA
Validated Target Region against Mouse SCN3A
GTTCGACCCTGACGCCACC
rcAAV Assay via PCR
PCR and sequencing. The following primers were used for PCR amplification for analysis of rcAAV:…
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