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Optimal MOIs for Some Cells Infected with Lentivirus

Cell line Tissue Cancer/cell type Species MOI
A431 Epithelial Carcinoma Human 5
A549 Lung Carcinoma Human 5
Astrocytes Nervous system Primary Human 1
B16-F10 Epithelial Melanoma, metastatic Mouse 5
BMM Bone Marrow Primary Human 8
BxPC-3 Pancreas, epithelial Adenocarcinoma Human 10
H3255 Lung Carcinoma, NSCLC Human 10
HCT116 Colon Carcinoma Human 5
HeLa Cervix Carcinoma, epitheloid Human 3
HEK293T Kidney Tumor Human 5
Hepa1-6 Liver Carcinoma Mouse 3
HMVEC Endothelial Endothelial, microvascular Human 100
HT-29 Colon Adenocarcinoma Human 3
HUVEC Umbilicus Endothelial cells Human 100
Jurkat Blood Leukemia, Acute T Cell Human 10
LLC-1 Lung Carcinoma Mouse 6
LNCaP Prostate Carcinoma Human 5
MM200 Skin Melanoma Human 5
MCF-7 Breast Adenocarcinoma Human 2
MDA-MB-231 Breast Adenocarcinoma Human 1
MM-AN Skin Melanoma, metastatic Human 16
MMC Breast Carcinoma Mouse 4
MRC-5 Lung, embryonic Fibroblasts Human 1
NB4 Blood Leukemia, acute promyelocytic Human 10
PC12 Adrenal gland Pheochromocytoma Rat 20
SKOV-3 Ovary Adenocarcinoma Human 15
U-2 OS Bone Osteosarcoma Human 5
Primary neurons Neuron N/A Mouse 20~30
Neuronal stem cell Stem cell N/A Mouse 20~100

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