LumiGOLD™ ECL Western Blotting Detection Kit (Ver. II)

Application: Substrate of Horseradish peroxidase for chemiluminescent detection of protein in Western Blot

Features:
▪ Chemiluminescence lasts for up to 6 hours without significant attenuation
▪ Extremely sensitive able to detect 0.1 pg protein with very low background
▪ Extremely stable for storage up to 12 mouths at 4 °C
▪ Working solution stable up to 72 hours at 4 °C

Comparisons of LumiGOLD™ ECL Western Blotting Detection Kit with Other Leading Brand Products:
A. LumiGOLD™ ECL Western Blotting Detection of EGFP (enhanced green fluorescent protein) in Wide Linear Range*




* 10 two-folder serial dilutions of purified EGFP (0.1 pg~51.2 pg in lane 1~10 from left to right, upper panel). EGFP was separated by SDS-PAGE electrophoresis followed by transfer to a PVDF membrane. The membrane was blocked with 5% skim milk (product # SL100317) and washed with TBST followed by incubation of mouse anti-EGFP monoclonal antibody. HRP-conjugated goat anti-mouse IgG was used to recognize the mouse anti-GFP. The blot was exposed to Fuji film for 20 seconds. The linear range of LumiGOLDD™ ECL kit was determined (lower panel) by addition of 10 two-folder serial diluted horseradish peroxidase (HRP) to the LumiGOLD™ working solution followed by determination of luminosity by Beckman Coulter LD400 luminometer

B. A comparison of LumiGOLD™ ECL Western Blotting Detection Kit with Popular Brand Products

* Noise refers to the background chemiluminescence of the working solution without addition of horseradish peroxidase (HRP)
** Signal refers to the HRP catalyzed chemiluminescence of the working solution after addition of 5 µl x HRP-conjugated goat anti-mouse IgG (100,000 dilution). Luminosity was determined by a Beckman Coulter LD400 luminometer

C. LumiGOLD™ ECL Western Blotting Detection of beta-Actin in Comparison of Amersham ECL Kit*

           Amersham ECL™                   LumiGOLD™



* HEK293 cell lysate was prepared with CompLysis™ Protein Extraction Reagent (Cat # SL100319) and the same amount of total proteins were loaded to the corresponding wells (lane #1=lane #5 / lane #2=lane #6 / lane #3=lane #7 / lane #4=lane #8). beta-Actin was picked up by anti-actin antibody followed by development with ECL reagents for 2 minutes. The exposure time was labeled in the above images

Data Sheet

Testimonials
I have used it for several times, it is good according to the results. It seems that this product is better than others to some degree...
------by Dr. Aibing Wang, NHLBI, NIH