Product Category: Pre-made
recombinant lentivirus;
LV-Synapsin-ChR2-Venus; LV-Syn-ChR2-Venus;
Syn-ChR2-Venus Lentivirus;
Description: LV-Syn-ChR2-Venus
is
a pre-made lentivirus which
over-expresses Channelrhodopsin-2
tagged with Venus
under the human Synapsin promoter. Channelrhodopsins are a subfamily of opsin proteins that
function as light-gated ion channels and very useful for many bioengineering
and neuroscience applications such as photostimulation of neurons for probing
of neural circuits. Using a Venus tagged ChR2, light-stimulated axons and
synapses can be identified in intact brain tissue. This is useful to study the
molecular events during the induction of synaptic plasticity. ChR2 has also
been used to map long-range connections from one side of the brain to the
other, and to map the spatial location of specific inputs on the dendritic
tree of individual neurons.
Titer: >
1x109 TU/mL
Selection Antibiotic: None
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2
x 25 µL (Std
Pack)
Storage Buffer: PBS
Infection Protocol:
Transfer Vector Map:
* Concentrated
via PEG precipitation followed by
ultra-centrifugation at 25000 rpm