Pre-made recombinant lentivirus; LV-CAG-hGluc-Puro; LV-CAGapsin-hGluc-Puro; LV-CAG-Gluc-IRES-Puro; LV-CAGapsin-hGluc-IRES-Puro; CAG-hGluc-Puro Lentivirus
Product Category: Pre-made
recombinant lentivirus; LV-CAG-hGluc-Puro; LV-CBA-hGluc-Puro; LV-CAG-Gluc-IRES-Puro; LV-CAG-hGluc-IRES-Puro; CAG-hGluc-Puro
Lentivirus
Description: LV-CAG-hGluc-Puro is
a pre-made lentivirus which over-expresses codon-optimized/humanized
Gaussia luciferase (hGluc) under
the CAG (also known as CBA) promoter with co-expression of puromycin selection in a IRES
cassette. The
humanized form of Gaussia luciferase (hGLuc) was confirmed to be nontoxic and
naturally secreted after over-expression in mammalian cells. hGLuc generated
over 1000-fold higher bioluminescent signal intensity from live cell and over
100-fold higher intensity from viable cells alone (not including secreted
luciferase) or cell lysates, compared to humanized forms of firefly (hFLuc) and
Renilla (hRLuc) luciferases expressed under similar conditions. Furthermore,
hGLuc showed 200-fold higher signal intensity than hRLuc and intensity
comparable to that of hFLuc in vivo under standard imaging conditions. Gaussia
luciferase provides a sensitive means of imaging gene delivery and other events
in living cells in both tissue culture and in vivo, with a unique combination of
features including high signal intensity, secretion, and ATP independence, thus
being able to report from the cells and their environment in real time. Ready
to use format.
Titer: >
1x109 TU/mL
Selection Antibiotic: Puromycin
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2
x 25 µL (Std Pack)
Storage Buffer: PBS
Infection Protocol:
Transfer Vector Map:
* Concentrated
via PEG precipitation followed by ultra-centrifugation
at 25000 rpm