Pre-made AAV, AAV1-Cre
Pre-made recombinant AAV; AAV-CAG-iCre; AAV-CBA-iCre; AAV8-CAG-iCre; AAV8-CBA-iCre
Product Category:
Pre-made recombinant
AAV; AAV-CAG-iCre; AAV-CBA-iCre; AAV8-CAG-iCre; AAV8-CBA-iCre
Description:
Cre
recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the
site-specific recombination of DNA between loxP sites. Cre recombinase is used
as a tool to genetically modify genes, such as to delete a segment of DNA
flanked by LoxP sites in cells or experimental animals. By optimizing the
mammalian codon usage for Cre recombinase, Cre expression is improved in the
mammalian cells. This codon-improved Cre (known as iCre) transgene shows lower
CpG content from the prokaryotic coding sequence, thus reducing the chances of
epigenetic silencing in mammals.
AAV8-CAG-iCre is
a pre-made rAAV in serotype 8 (with capsid from AAV8 and 2xITR
from AAV2) which expresses iCre recombinase under CAG promoter (also known as
CBA promoter).
This product used in the Cre-lox system as a genetic tool to generate
site-specific recombination of DNA between loxP sites in cultured cells and
animal experiments.
Titer:
> 1x1013 VG/mL
Size:
>
30 µL (Std Pack)
Storage Buffer:
PBS
Infection Protocol:
Vector Map:
