Production of Recombinant Pseudotyped Lentivirus with Lenti.MAX Packaging System:
- Derived from 3rd generation packaging system with a shRNA construct included for maximum yield.
- An inhibitory trans element upstream of 5'-LTR allows packaging toxic transgenes.
- LentiMAX™ packaging cell line is optimized for high pseudoviral titers.
Large lentivirus trans vector inventory for constructing lentivector carrying a specific promoter and reporter:
Service Description:
1. Clone your gene of interest (GOI) into a lentivirus trans vector.
2. Lentivirus packaging via Lenti.MAX™ packaging system..
3. Lentivirus purification via PEG precipitation followed by ultra-centrifugation.
4. Recombinant lentivirus titration via p24 ELISA.
Required Material: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).
Turnaround Time: ~2 weeks.
Deliverables: 5.0 mL (20x0.25 mL) at >10^9 TU/mL* in vivo grade** super purified lentiviral vector.
We offer discount for new customer, please request a quote with us today.
We offer truncated service as well, please request a quote with us today.
* Despite the fact that the final lentivirus yield depends on the nature of transgene and varies from batch to batch, we guarantee the titer >2x10^9 TU/mL.
** We deliver in vivo grade of lentivirus which is OK for both in vitro tissue culture infection and in vivo injection.