> shRNA/miRNA AAV Service

shRNA/miRNAgRNA AAV Production Service

shRNA/miRNA/gRNA AAV Production Service:

Adeno-associated virus (AAV) is a small virus (20 nm in diameters) replication-defective, nonenveloped virus, which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell.

With our proprietary genetically engineered AAV·HT™ packaging cell, we are offering custom shRNA/miRNA/gRNA rAAV vector production services (conventional single stranded or double-stranded also known as self-complementary) listed below to fit your different research needs.

Catalog #

Service Items

Final Deliverable *

rAAV Purity

Lead Time

SL100810

shRNA/miRNA/gRNA rAAV Production, Full Service, Large Scale

1.0 mL at >1E+13 VG/mL

In Vivo Grade

3~4 weeks

SL100871

shRNA/miRNA/gRNA rAAV Production, Full Service, Medium Scale

0.2 mL at >1E+13 VG/mL

In Vivo Grade

3~4 weeks

SL100874

shRNA/miRNA/gRNA rAAV Production, Full Service, Pilot Scale

0.1 mL at >1E+13 VG/mL

In Vivo Grade

3~4 weeks

SL100872

shRNA rAAV Packaging Service, Large Scale

1.0 mL at >1E+13 VG/mL

In Vivo Grade

1~2 weeks

SL100873

shRNA rAAV Packaging Service,
Medium Scale

0.2 mL at >1E+13 VG/mL

In Vivo Grade

1~2 weeks

SL100875

shRNA/TuD/gRNA Validation Service**

>6xshRNA rAAV cis Plasmids with >1 Plasmid at 80% KD

N/A

2~3 weeks



Large shRNA/miRNA/gRNA AAV cis vector inventory for constructing AAV cis plasmid with a specific promoter and reporter:

 
 For Conventional shRNA/miRNA/TuD rAAV Production

 Single-promoter shRNA/miRNA AAV cis vector

 Dual-promoter shRNA/miRNA rAAV cis vector

             Promoterless
             CMV
             CAG
             H1
            
U6
             Synapsin
             UBC

             EF1α
             ALB(1.4)
             ApoE/AAT1
             CaMKII
             ELA1
             Enh358MCK
             cTNT
             GFAP
             MBP
             SST
             TBG

             αMHC
             hRPE
             mIP1
             tMCK





















 

             Promoterless
             CMV
             CAG
             H1
            
U6
             Synapsin
             UBC

             EF1α
             ALB(1.4)
             ApoE/AAT1
             CaMKII
             ELA1
             Enh358MCK
             cTNT
             GFAP
             MBP
             SST
             TBG

             αMHC
             hRPE
             mIP1
             tMCK

  CMV
  CAG
  H1
 
U6
  Synapsin
  UBC

  EF1α
  CaMKII
  cTNT
  GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 

 
 For CRISPR/Cas9 rAAV Production

 Single-promoter CRISPR/Cas9 AAV cis vector

 Dual-promoter CRISPR/Cas9 AAV cis vector

      Promoterless
      U6
      H1
      CMV
      CAG
      CBH
      Synapsin
      UBC

      EF1α
      ALB(1.4)
      ApoE/AAT1
      CaMKII
      ELA1
      Enh358MCK
      cTNT
      GFAP
      MBP
      SST
      TBG

      αMHC
      hRPE
      mIP1
      tMCK

  Empty
  CBH
  U6
  H1
  sCMV
  Syn
  sEF1α
  CK 0.4
  cTNT
  GFAP 0.6
  sSyn
  SST
  TBG
  αMHC
  hRPE
  mIP1
  tMCK
  CBH

Cas9
hCas9

SpCas9
hSpCas9
SaCas9
AsCpf1
hAsCpf1
ftCas9
st1Cas9
cjCas9
cdCas9
ciCas9
ncCas9
st3Cas9
nmCas9
mgCas9
nsCas9

 

      Promoterless
      sCMV
      U6
      H1
      tRNA
      Syn
      sSyn
     
sEF1α
      CaMKII 0.4

  CMV
  sCMV
  CBH
  H1
 
U6
  Syn
  sSyn

  sEF1α
  CaMKII 0.4





 

Cas9
hCas9

SpCas9
hSpCas9
SaCas9
AsCpf1
hAsCpf1
ftCas9
st1Cas9
cjCas9
cdCas9
ciCas9
ncCas9
st3Cas9
nmCas9
mgCas9
nsCas9


 


Required Materials: GenBank access #, target sequence, or validated siRNA, shRNA sequence; miRNA accession # for TuD, pre-miRNA or mature miRNA or gRNA sequence

Deliverables: Total >1.0 ml at >1013 VG/mL*** shRNA/miRNA/gRNA rAAV for large scale production.

With our proprietary genetically engineered AAV·HT™ packaging cell and a modified rAAV cis plasmid, the shRNA/miRNA rAAV yield is easy to reach super high level--------total 1E+15 VG.

For super high level rAAV production up to 1E+15 VG, please contact us to request a quote.


* Some terms may apply for custom double-stranded or self-complementary rAAV production service.
** If commercial shRNAs or siRNAs, gRNAs of your target gene or tough decoy (TuD) miRNA are not available or non-functional, or your project requires special design for further development, we offer special shRNA/gRNA/TuD design without additional charge!
*** Final viral yield may depend on the transgene in the virus.



Catalog # Product Name+ Price
SL100872

shRNA/miRNA rAAV Packaging, Large Scale

shRNA/miRNA AAV Packaging in Large Scale Features: - Excellent gene delivery efficiency in most cell...



... more info


SL100873

shRNA/miRNA rAAV Packaging, Medium Scale

shRNA/miRNA AAV Packaging in Medium Scale Features: - Excellent gene delivery efficiency in most cell...



... more info


SL100875

shRNA/miRNA Validation Service

shRNA/miRNA Validation Service shRNA/miRNA Validation Service via Cell-based Reporter System: - Cloning...



... more info


SL100810

shRNA/miRNA/gRNA rAAV Production Full Service, Large Scale

shRNA/miRNA/gRNA rAAV Production Full Service, Large Scale Features: - Excellent gene delivery efficiency...



... more info


SL100871

shRNA/miRNA/gRNA rAAV Production Full Service, Medium Scale

shRNA/miRNA/gRNA rAAV Production Full Service, Medium Scale Features: - Excellent gene delivery...



... more info


SL100874

shRNA/miRNA/gRNA rAAV Production Full Service, Pilot Scale

shRNA/miRNA/gRNA rAAV Production Full Service, Pilot Scale Features: - Excellent gene delivery efficiency...



... more info




Copyright © 2017 SignaGen Laboratories. All Rights Reserved.