shRNA/miRNA/gRNA Adenovirus Production Full Service Pilot Scale


  • Catalog #: SL100785
  • Unit Size: 1.0 mL at >5E+10~5E+11 PFU/mL

shRNA/miRNA/gRNA Adenovirus Production Full Service Pilot Scale

Production of Recombinant Serotype 5 shRNA/miRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
- One-stop service: from shRNA/miRNA design to packaging.
- Unsurpassable transduction efficiency.
- Quick turnaround time: 3 to 4 weeks.
- Cost-effective.
- Special design request acceptable, FREE!


Large shRNA/miRNA/gRNA Ad.MAX™ shuttle vector collection for cloning shuttle plasmid with a specific promoter and reporter:

 Single-promoter Ad.MAX™ shuttle vector

 Dual-promoter Ad.MAX™ shuttle vector

            Promoterless
            CMV
            CAG
            H1
           
U6
            Synapsin
            UBC

            EF1α
            ALB(1.4)
            ApoE/AAT1
            CaMKII
            ELA1
            Enh358MCK
            cTNT
            GFAP
            MBP
            SST
            TBG

            αMHC
            hRPE
            mIP1
            tMCK



















 

            Promoterless
            CMV
            CAG
            H1
           
U6
            Synapsin
            UBC

            EF1α
            ALB(1.4)
            ApoE/AAT1
            CaMKII
            ELA1
            Enh358MCK
            cTNT
            GFAP
            MBP
            SST
            TBG

            αMHC
            hRPE
            mIP1
            tMCK

CMV
CAG
H1

U6
Synapsin
UBC

EF1α
CaMKII
cTNT
GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


Service Description:
1. Synthesize and clone shRNA/miRNA to a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2.
Viral production in Ad.MAX™ 293 cells: transfection and purification of recombinant adenovirus.
3. Generation of high titer recombinant adenoviral stock in ~500 cm2 Ad.MAX™ 293 cells.
4. Recombinant adenovirus PFU titration.


Required Materials: NCBI accession # of target gene, target region sequence, shRNA or siRNA sequence validated/published or mature miRNA sequence, miRNA accession # or gRNA sequence etc.

Turnaround Time: 3 ~ 4 weeks.

Deliverables: 1.0 mL (5x0.2 mL) of super infectious in vitro grade adenovirus vector at 5E+10~5E+11 PFU/mL*.

We offer discount for new customer, please request a quote with us today.

We also offer truncated custom shRNA/miRNA/gRNA adenovirus service, please
request a quote with us today.


* Final viral yield may depend on the transgene in the virus.
 




Copyright © 2017 SignaGen Laboratories. All Rights Reserved.