shRNA/miRNA/gRNA Adenovirus Production, Full Service Medium Scale


  • Catalog #: SL100781
  • Unit Size: 2.0 mL at 5E+10~5E+11 PFU/mL

shRNA/miRNA/gRNA Adenovirus Production, Full Service Medium Scale

Production of Recombinant Serotype 5 shRNA/miRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
- One-stop service: from shRNA/pre-miRNA design to packaging.
- Unsurpassable transduction efficiency.
- Quick turnaround time: 3 to 4 weeks.
- Cost-effective.
- Special design request acceptable, FREE!


Large shRNA/miRNA/gRNA Ad.MAX™ shuttle vector collection for cloning shuttle plasmid with a specific promoter and reporter:

 Single-promoter Ad.MAX™ shuttle vector

 Dual-promoter Ad.MAX™ shuttle vector

            Promoterless
            CMV
            CAG
            H1
           
U6
            Synapsin
            UBC

            EF1α
            ALB(1.4)
            ApoE/AAT1
            CaMKII
            ELA1
            Enh358MCK
            cTNT
            GFAP
            MBP
            SST
            TBG

            αMHC
            hRPE
            mIP1
            tMCK



















 

            Promoterless
            CMV
            CAG
            H1
           
U6
            Synapsin
            UBC

            EF1α
            ALB(1.4)
            ApoE/AAT1
            CaMKII
            ELA1
            Enh358MCK
            cTNT
            GFAP
            MBP
            SST
            TBG

            αMHC
            hRPE
            mIP1
            tMCK

CMV
CAG
H1

U6
Synapsin
UBC

EF1α
CaMKII
cTNT
GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


Service Description:
1. Design, synthesize and clone shRNA/miRNA/gRNA to a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Initial adenoviral stock production.
3. Adenovirus amplification from ~1000 cm2 Ad.MAX™ 293 cells.
4. Recombinant adenovirus PFU titration.


Required Materials: NCBI accession # of target gene, target region sequence, shRNA or siRNA sequence validated/published or mature miRNA sequence, miRNA accession # or gRNA sequence  etc.

Turnaround Time: 3 ~ 4 weeks.

Deliverables: 2 mL (10x0.2 mL) of super infectious in vitro grade adenovirus vector at 5E+10~5E+11 PFU/mL.

We offer discount for new customers, please request a quote with us today.

We also offer truncated shRNA/miRNA service, please
request a quote with us today.



* Final adenovirus yield may depend on the nature of transgene.
 



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