GenJet™ In Vitro DNA Transfection Reagent for Primary Macrophages

Price: $298.00

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  • Catalog #: SL100489-PMG
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for Primary Macrophages is pre-optimized for transfecting primary macrophages. Macrophages are white blood cells within tissues, produced by the differentiation of monocytes. Monocytes and macrophages are phagocytes, acting in both non-specific defense (innate immunity) as well as to help initiate specific defense mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytose (engulf and then digest) cellular debris and pathogens either as stationary or as mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen. They can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), Lysozyme M, MAC-1/MAC-3 and CD68 by flow cytometry or immunohistochemical staining.  GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

 

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~70%
PRMI with 10% FBS
3:1
1xGenJet™ Transfection Buffer
15 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter)
75%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol




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