GenJet™ In Vitro DNA Tranfection Reagent for C2C12 Cells

Price: $156.00

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  • Catalog #: SL100489-C2C12
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Tranfection Reagent for C2C12 Cell is pre-optimized for transfecting C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).

Refer to the following optimal transfection conditions for maximal transfection efficiency on C2C12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Shaved CellTransfection Conditions:
Cell culture medium
Confluency of cell on the day of shaving
Cell density for plating

GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Incubation time of shaved cells with transfection complex
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


DMEM with 4.5 g/L glucose, 10% FBS

95~100%
1.3x10^5 cells per square c
entimeter
4:1
Serum-free DMEM with 4.5 g/L glucose

12 minutes at RT
20 minutes at 37 degree
48 hours


EGFP
pEGFP-N3 (CMV promoter)

65%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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