Description
GenJet™ DNA In Vitro
Transfection Reagent for
C2C12 Cell is pre-optimized for transfecting C2C12 cells. C2C12 cells were
originally obtained by Yaffe and Saxel (1977) through selective serial passage
of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush
injury. These cells were shown to be capable of differentiation. C2C12 cells are
a useful model to study the differentiation of non-muscle cells to skeletal
muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins
and the androhen receptor (AR).
Refer to the following optimal transfection conditions for maximal transfection
efficiency on C2C12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of
Optimal Shaved CellTransfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of shaving
Cell density for plating
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Incubation time of shaved cells with transfection complex
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
DMEM with 4.5 g/L glucose, 10% FBS
95~100%
1.3x10^5 cells per square centimeter
4:1
Serum-free DMEM with 4.5 g/L glucose
12 minutes at RT
20 minutes at 37 degree
48 hours
EGFP
pEGFP-N3 (CMV promoter)
65% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
