LipoJet™ In Vitro Transfection Kit (Ver. II)

Description:
Based on our innovative and proprietary lipid-conjugation technology, LipoJet™ Transfection Kit, formulated from novel fluorinated cationic lipids, exhibits significant difference from other lipids transfection reagents in the market. LipoJet™ Transfection Kit is the most powerful gene delivery tool for a variety of applications including plasmid DNA, siRNA and shRNA transfections and DNA/siRNA co-transfection.

Kit content:
- LipoJet™ Reagent, 1.0 ml, sufficient for ~667 DNA transfections (0.5 µg DNA per well of 24-well plate) and ~1333 siRNA transfections (final 5.0 nM siRNA per well of 24-well plate)
- LipoJet™ Transfection Buffer(5x ), formulated for maximal transfection efficiency, 8.0 ml at 5x concentrated stock solution to make 40 ml of working solution

Features:
- Best for broad mammalian cells
- 95% silencing at 5.0 nM siRNA
- No sera interference
- Simple and easy protocol
- Suitable for high-throughput application

Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Examples Showing Excellent DNA and siRNA Transfection Efficiency of LipoJet™ In Vitro Transfection Kit

Superior DNA transfection efficiency of LipoJet™ reagent. pEGFP-N3 cDNA (0.5 µg per well of 24-well plate) was delivered into HEK293 (upper panel) and Hela (lower panel) cells with LipoJet™ (left panel) and PolyJet™ (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection




Exceptional gene silencing of LipoJet™ reagent during DNA/siRNA co-transfection on mammalian cells. Transfection of GFP cDNA (left panel, 0.25 µg per well of 24-well plate) and co-transfection of GFP cDNA ( 0.25 µg per well of 24-well plate) and GFP targeted siRNA (final 1.0 nM, right panel) with LipoJet™ reagent leads to exceptional gene silencing on HEK293 (upper panel), Hela (middle panel) and SaoS-2 (lower panel) cells respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection



Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with 1.0 µl of LipoJet™ reagent and 0.5 pmol EG5 siRNA per well of 24-well plate. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 1.0 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 1.0 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.


Data Sheet & Protocol:
- A Protocol for DNA Transfection
- A Protocol for siRNA Transfection
- A Protocol for DNA/siRNA Co-transfection

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