GenJet™ In Vitro DNA Transfection Reagent for MCF 10A Cell

Price: $198.00

Add to Cart:

  • Catalog #: SL100489-MCF10A
  • Unit Size: 1.0 mL

Please Choose:



Description
GenJet™ DNA In Vitro Transfection Reagent for MCF 10A Cell is pre-optimized for transfecting the non-tumorigenic epithelial cell line, MCF 10A. The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.  They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence.  The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).  By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. With our proprietary transfection booster conjugation technology, this reagent was formulated to transfect MCF 10A cells with up to 50% efficiency (GFP positive cells).
 

Summary of Optimal Shaved Cell Transfection Conditions:
Cell culture medium
Confluency of cell on the day of shaving
Cell density for plating

GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Incubation time of shaved cells with transfection complex
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


DMEM/F12 with 5% HS and other additives
95~100%
1.3x10^5 cells per square c
entimeter
4:1
Serum-free DMEM/F12

15 minutes at RT
20 minutes at 37 degree
48 hours


EGFP
pEGFP-N3 (CMV promoter)

50%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet

To request a free trial sample, please Create An Account with us to enter your shipping address and email us at order@signagen.com




Copyright © 2017 SignaGen Laboratories. All Rights Reserved.