GenJet™ In Vitro DNA Transfection Reagent for RAW 264.7 Cell

Price: $298.00

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  • Catalog #: SL100489-RAW
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for RAW 264.7 Cell is pre-optimized for transfecting RAW 264.7 cells.  RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. RAW 264.7 cell slide is often used for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

 

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~80%
DMEM with 10% FBS
3:1
1xGenJet™ Transfection Buffer
10 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter)
75%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol




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