TransMate™ Transfection Booster Cocktail

Price: $128.00

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  • Catalog #: SL100498
  • Unit Size: 1.0 ml

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Description:
HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity.  Incubation of sensitive cells (e.g., SaoS-2, HUVEC, primary kerotinocytes, etc) with HapiCell Toxicity Removal Reagent for 2 minutes at RT was confirm to completely remove the transfection related toxicity which otherwise kill the cells. HapiCell™ Transfection Toxicity Removal Cocktail, 125 ml, is sufficient to treat 125 wells for 24-well plate and 63 wells for 6-well plate respectively.

Feature:
- Removes transfection meidated toxicity after 2 minutes incubation with cells at RT
- Remove extracellular complexes of DNA and cationic lipids or polymers
- Nontoxic to cells
- Maintain cell viability

HapiCell™ Transfection Toxicity Removal Cocktail Effectively Remove Transfection Mediated Toxocity:

A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on mouse skin fibroblast.  A DNA encoding GFP was delivered to mouse skin fibroblasts by Lipofectamine 2000 in absence of serum.  Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (right panel) or without (left panel) HapiCell™ Transfection Toxicity Removal Cocktail.  The cell viability was checked under microscope 24 hours post transfection


A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on primary mouse kerotinocytes.  A DNA encoding GFP was delivered to primary mouse kerotinocytes by Lipofectamine 2000 in absence of serum.  Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (right panel) or without (left panel) HapiCell™ Transfection Toxicity Removal Cocktail.  The cell viability was checked under microscope 24 hours post transfection

Data Sheet
 




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