GenJet™ In Vitro DNA Transfection Reagent for Ca Ski Cells

Price: $178.00

Add to Cart:

  • Catalog #: SL100489-CaS
  • Unit Size: 1.0 ml

Please Choose:



Description

GenJet™ DNA In Vitro Transfection Reagent for Ca Ski Cell is pre-optimized for transfecting Ca Ski Cells, human cervix epidermoid carcinoma cells.  Refer to the following optimal transfection conditions for maximal transfection efficiency on Ca Ski cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, 18~24 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter)

88%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




Copyright © 2024 SignaGen Laboratories. All Rights Reserved.