Description
GenJet™ DNA In Vitro
Transfection Reagent for NMuMG cells is pre-optimized for transfecting NMuMG cells.
NMuMG is mouse mammary gland, normal epithelial. NMuMG cells express
different integral surface proteoglycans (e.g. syndecan). They are used for
studies of cell adhesion and growth factor-binding molecules as well as for
analysis of growth factor effects on cell proliferation.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on NMuMG cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~90%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
No
48 hours
EGFP
pEGFP-N3 (CMV promoter)
75% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on NMuMG Cells. NMuMG cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
