In Stage 1, Set to 2 min at 25°C; 1 Rep.In Stage 2, Set to 15 min at 55°C; 1 Rep.In Stage 3, Set to 2 min at 95°C, 1 […]
Validated Target Region against Mouse PTBP1 Gene
5′-GGGTGAAGATCCTGTTCAATA-3′
Research Use Only 2019-Novel Coronavirus (2019-nCoV, Covid-19) Real-time RT-PCR Primers and Probes
Name Description Oligonucleotide Sequence (5’>3’) Label1 FinalConc. 2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer GAC CCC AAA ATC AGC GAA AT None 500nM 2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer TCT GGT TAC TGC CAG TTG […]
Bmi-1 Validated target Regions
GAGATAATAAGCTTGTCTA ACAACCAGAATCAAGATCA ATATGGATGTTAAGTGGAA
Validated siRNA Targeting Mouse Trpv1 Gene
5’-GTCGGTTTATGTTCGTCTAC-3’
A Novel AAV Serotype B1 for CNS Transduction
VP1 Amino Acid Sequence of AAV-B1: MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHAD VEFQERLQEDTSFGGNLGRAVFQAKKRVLEPFGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVG PNTMAAGGGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRD WQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYF PSQMLRTGNNFEFSYQFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTARTQQLLFSQAGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNN SNSAWTAGTKYHLNGRNSLANPGIAMATHKDDEERFFPSNGILIFGKQNAARDNADYSDVMLTSEEEIKTTNPVATEEYGIVADNLQQQNTAPQIGTVNSQ GALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEI QYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL
Novel AAV-F AAV-S for Neuronal Cortex Transduction
AAV-F and AAV-S were confirmed to mediate transgene expression in the brain cortex more than 65-fold (astrocytes) and 171-fold (neurons) higher than the parental AAV9. The VP1 amino acid sequence […]
Virus used in gene therapies may pose cancer risk, dog study hints
Just as gene therapy finally seems to be living up to its promise, a study has revived a lingering worry about the viral vessel that many efforts rely on to […]
Why do I see amplification curves in my NTC samples?
Products can appear in non-template control (NTC) wells due to the ability of some primer sequences to form structures that can be used as substrates by DNA polymerases. The most common […]