1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg 121 gccaactcca tcactagggg ttcctggagg ggtggagtcg tgacgtgaat tacgtcatag 181 ggttagggag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt gcgacaccat 241 gtggtcacgc […]
Adeno-associated Virus (AAV) - 17. page
AAV2 Capsid Protein VP3
10 20 30 40 50 MATGSGAPMA DNNEGADGVG NSSGNWHCDS TWMGDRVITT STRTWALPTY 60 70 80 90 100 NNHLYKQISS QSGASNDNHY FGYSTPWGYF DFNRFHCHFS PRDWQRLINN 110 120 130 140 150 NWGFRPKRLN FKLFNIQVKE VTQNDGTTTI ANNLTSTVQV FTDSEYQLPY […]
AAV2 Capsid Protein VP2
10 20 30 40 50 MAPGKKRPVE HSPVEPDSSS GTGKAGQQPA RKRLNFGQTG DADSVPDPQP 60 70 80 90 100 LGQPPAAPSG LGTNTMATGS GAPMADNNEG ADGVGNSSGN WHCDSTWMGD 110 120 130 140 150 RVITTSTRTW ALPTYNNHLY KQISSQSGAS NDNHYFGYST PWGYFDFNRF […]
AAV2 Capsid Protein VP1
10 20 30 40 50 MAADGYLPDW LEDTLSEGIR QWWKLKPGPP PPKPAERHKD DSRGLVLPGY 60 70 80 90 100 KYLGPFNGLD KGEPVNEADA AALEHDKAYD RQLDSGDNPY LKYNHADAEF 110 120 130 140 150 QERLKEDTSF GGNLGRAVFQ AKKRVLEPLG LVEEPVKTAP GKKRPVEHSP […]
Capsid mutation(s) of rAAV leads to enhanced transduction efficiency
AAV Serotype Capsid Mutation(s) AAV2 Y730F AAV2 Y444F AAV2 Y444F+Y730F AAV2 Y444F+Y730F+Y500F AAV2 Y444F+Y730F+Y500F+Y272F AAV8 Y733F AAV8 Y447F AAV9 Y446F AAV9 Y731F AAV9 Y446F + Y731F
AAV 2 vector: Point mutations in tyrosines lead to high-efficiency transduction.
AAV2-mediated transduction of hepatocytes from normal C57BL/6 mice injected via tail vein with tyrosine-mutant capsid scAAV2-EGFP vectors. (A) Transgene expression was detected by fluorescence microscopy 2 weeks after injection of […]
Suppression of microRNA via Tough Decoy rAAV
http://signagen.com/Application_Notes/Application_Note_AAV_2015_9.pdf
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]
Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG […]