10 20 30 40 50 MAPGKKRPVE HSPVEPDSSS GTGKAGQQPA RKRLNFGQTG DADSVPDPQP 60 70 80 90 100 LGQPPAAPSG LGTNTMATGS GAPMADNNEG ADGVGNSSGN WHCDSTWMGD 110 120 130 140 150 RVITTSTRTW ALPTYNNHLY KQISSQSGAS NDNHYFGYST PWGYFDFNRF […]
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AAV2 Capsid Protein VP1
10 20 30 40 50 MAADGYLPDW LEDTLSEGIR QWWKLKPGPP PPKPAERHKD DSRGLVLPGY 60 70 80 90 100 KYLGPFNGLD KGEPVNEADA AALEHDKAYD RQLDSGDNPY LKYNHADAEF 110 120 130 140 150 QERLKEDTSF GGNLGRAVFQ AKKRVLEPLG LVEEPVKTAP GKKRPVEHSP […]
AAV 2 vector: Point mutations in tyrosines lead to high-efficiency transduction.
AAV2-mediated transduction of hepatocytes from normal C57BL/6 mice injected via tail vein with tyrosine-mutant capsid scAAV2-EGFP vectors. (A) Transgene expression was detected by fluorescence microscopy 2 weeks after injection of […]
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]
Search a promoter for a specific gene
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Eukaryotic Promoter Database
EPDnew (Eukaryotic Promoter Database) – is a new collection of experimentally validated promoters in human, mouse, D. melanogaster and zebrafish genomes. Evidence comes from TSS-mapping from high-throughput expreriments such as […]
Common promoters for eukaryotes and prokaryotes
Eukaryotic Promoters Promoter Primarily used for RNA transcript Description Expression Additional considerations CMV General expression mRNA Stong mammalian expression promoter from the human cytomegalovirus Constitutive May contain an enhancer region. […]
Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG […]
Comparison of EF-1 alpha and CMV promoterd for engineering stable tumor cell lines using rAAV
Stable HT-29 clones with > 97% of all cells homogeneously expressing GFP were generated with the EF-1 alpha promoter. In contrast in clones carrying the CMV promoter, only up to […]