Monthly Archives: January 2018

COX-2 siRNA: 5-AACTGCTCAACACCGGAATTTTT-3 COX-1 siRNA: 5-AAGTGCCATCCAAACTCTATCTT-3 15-PGDH siRNA: 5-AACAGTGGTGTGAGACTGAAT-3

15-PGDH and Scramble control siRNA sequence: 15-PGDH: (NM_001034419.2) 5′-GCUUUGAA UGGUGCUAUUATT-3′ Scramble: 5′-UUCUCCGAACGUGUCAC GUTT-3′ HIF-1α siRNA: (NM_174339.3) 5′-GCUCCUGAGGAAGAACUAATT-3′ Scramble: 5′-UUCUCCG AACGUGUCACGUTT-3′

15PGDH-specific shRNAs target region sequence: 1) CACAGCCATCCTTGAATCAAT 2) GAAGGCGGCATCATTATCAAT 3) GCAACAACTGAGAGACACTTT 4) CTGGAGTGAATAATGAGAAA 5) ACTCATAACAACACAGACATA

Mouse FOXO3 siRNA: 5′-CCAGUGACUUGGACCUGGACAUGUU-3′

Mouse FOXO1 siRNA 5′-GCGGGCUGGAAGAAUUCAATT-3′

Human FOXO1 siRNAs: 5′-GGAGGUAUGAGUCAGUAUATT-3′ (sense) 5 ′-UAUACUGACUCAUACCUCCTT-3 ′ (antisense),  

Human Period 2 (hPer2) target sequences were as follows: i) 5’-CAACAAGGTGCTGAGAGTCAGCTTT-3’ ii) 5’-ACTCTGGTTATGAAGCCCCTAGAAT-3’

Two validated Gadd45a siRNAs siRNA1: sense: 5′- GAG CAG AAG ACC GAA AGG AUG GAU A-3′; anti-sense: 5′-UAU CCA UCC UUU CGG UCU UCU GCU C-3′ siRNA2: sense 5′-GGU GAC […]