Pre-made recombinant lentivirus; LV-EF1α-SpCas9; LV-EF1α-Cas9; LV-EF1A-SpCas9; LV-EF1A-Cas9; EF1A-Cas9 Lentivirus
Product Category: Pre-made
recombinant lentivirus; LV-EF1α-SpCas9; LV-EF1α-Cas9; LV-EF1A-SpCas9; LV-EF1A-Cas9; EF1A-Cas9
Lentivirus
Description: LV-EF1α-SpCas9 is
a pre-made lentivirus which over-expresses SpCas9 under
the EF1α promoter
and can be used along with guided RNA for genome-editing. The Type II
prokaryotic CRISPR/Cas system is the new class of tools for targeted genome
engineering. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease
enzyme associated with Streptococcus pyogenes' CRISPR immunity system. The cas9
nucleases derived from clustered regularly interspaced short palindromic repeats
(CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a
sequence-specific manner. With its ease in designing guide sequences to target
specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and
robust alternative to TALEN and Zinc finger nuclease platforms.
Ready
to use format.
Titer: >
1x109 TU/mL
Selection Antibiotic: None
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2
x 25 µL (Std Pack)
Storage Buffer: PBS
Infection Protocol: 
Transfer Vector Map:
* Concentrated
via PEG precipitation followed by ultra-centrifugation
at 25000 rpm
