AAV-CAG-GCaMP6f (AAV Serotype 1)

Price: $488.00

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  • Catalog #: SL101472
  • Unit Size: 1 x >30 µL (>1E+13 VG/mL)

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Pre-made recombinant AAV; AAV-CAG-GCaMP6f; AAV-CBA-GCaMP6f; AAV5-CAG-GCaMP6f; AAV5-CBA-GCaMP6f

Product Category: Pre-made recombinant AAV; AAV-CAG-GCaMP6f; AAV-CBA-GCaMP6f; AAV1-CAG-GCaMP6f; AAV1-CBA-GCaMP6f

Description: 
GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase.  When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms.

GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level.  Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and medium - GCaMP6m). 

The AAV1-CAG-GCaMP6f is a pre-packaged rAAV in serotype 1 (with capsid from AAV serotype 1 and 2xITR from AAV serotype 2) which over-expresses genetically encoded calcium indicator GCaMP6f under CAG promoter (also known as CBA promoter).  

Titer: > 1x1013 VG/mL

Size: > 30 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 


Vector Map: 
Pre-made recombinant AAV; AAV-CAG-GCaMP6f; AAV-CBA-GCaMP6f; AAV1-CAG-GCaMP6f; AAV1-CBA-GCaMP6f




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