GenJet™ In Vitro DNA Transfection Reagent (Ver. II) is upgraded
version of GenJet™ In Vitro DNA Transfection Reagent. Adopting a new
chemistry, more DNA condensing groups were liberated in GenJet™ (Ver.
II) (100 % vs. 80% in old version), leading to 3~4 folds increase of
transfection efficiency without sacrificing cell viability. GenJet™
(Ver. II) reagent, 1.0 ml, is sufficient for ~667 transfections in 24
well plates or ~333 transfections in 6 well plates.
Features compared with old version GenJet™ In
Vitro DNA Trasnfection Reagent
Cell-dependent 3~4 times higher efficiency
- Top choice for hard-to-transfect cells
- Efficiency boosted in the presence of serum and antibiotics for most
of cell types
- Exceptional high levels of recombinant protein production
Store at 4 °C. If stored properly, the product is stable
for 12 months or longer.
Comparisons of Transfection Efficiency and Cytotoxicity of GenJet™
(Ver. II) with Old Version GenJet™ and Other Leading Products
of transfection efficiency (left panel) and toxicity (right panel)
GenJet™ Ver. II with old version GenJet™ on commonly used mammalian
cells. phRL-CMV encoding Renilla Luciferase was introduced into
cells with the presence of serum and antibiotics. The Renillia
Luciferase activity was determined with Renilla Luciferaase Assay
System (Promega). For cytotoxicity, MTT assay was employed to determine
cell viability with untransfected control as 100%.
comparison of transfection efficiency of GenJet™ (Ver. II) vs.
Lipofectamine 2000 on primary rat aortic smooth muscle cells. Primary
rat aortic smooth muscle cells were prepared and transfected with a
nuclear targeted GFP cDNA by GenJet™ (right panel) and Lipofectamine
2000 (L2K, left panel) respectively. The transfection efficiency were
checked under a Nikon Eclipse 2000 fluorescence microscopy 48 hours
Transfection efficiency comparison of GenJet™ Ver. II with old version
GenJet™ on 293T cells. pEGFP-N3 was transfected to 293T cells at 90%
confluency with presence of serum and antibiotics. GFP fluorescence was
detected with Nikon Eclipse 2000 microscopy 48 hours post transfection
Lower panel: Transfection efficiency comparison of GenJet™ Ver.
II with old version GenJet™ on Hela cells. pEGFP-N3 was transfected to
Hela cells at 90% confluency with presence of serum and antibiotics.
GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48
hours post transfection
Information & Datasheet
- A Short Protocol for Advanced Users
- A Protocol for Transfecting Suspension 293 and CHO Cells
- A Protocol for Lentivirus Production
- A Protocol rAAV Production
- Technical Note & Transfection Tips
GenJet™ In Vitro DNA Transfection reagent
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