Transfection Reagent Selection Chart

Transfection Reagent Selection Chart from SignaGen

In Vitro DNA Transfection Reagents:
Based on our unique and proprietary “VIPs” and polymer synthesis technologies, currently we are developing and manufacturing three categories of DNA/siRNA transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.

Transfection Reagent Selection Chart.

Reagents

Features

Transfection Type

Toxicity*

CalFectin™

- Traditional recipe with new formulation
- Most affordable and efficient
- Best for large scale virus, antibody and protein production

DNA
siRNA
DNA/siRNA

++++

GenJet™

- Low toxicity for very sensitive cells
- Maximum cell viability

DNA
siRNA
DNA/siRNA

++

GenJet™ (Ver. II)

- 100% DNA condensing groups released
- Efficient for commonly used cell types

DNA
siRNA
DNA/siRNA

+++

GenJet™ Plus

- Enhanced conjugate for maximal efficiency
- Extremely good for very long DNAs

DNA
siRNA
DNA/siRNA

++

LipoD293™

- Enhanced conjugate with liposome for maximal efficiency
- Best for lentivirus, antibody and protein production

DNA
siRNA
DNA/siRNA

+++

LipoJet™

- Fluorinated cationic lipids
-
Best transfection efficiency with least toxicity

DNA
siRNA
DNA/siRNA

+

PolyJet™

- Biodegradable polycationic polymer
- Excellent efficiency for common used mammalian cells

DNA
siRNA
DNA/siRNA

+++

* The cell viability was evaluated by MTT assay. Total 0.5 µg of pEGFP-C1 was transfected to Hela and HEK293T cells respectively on 24-well plate with different transfection reagents in comparison of Lipofectaminne 2000 (toxicity arbitrary grade  ++++) and Fugene 6 (toxicity arbitrary grade +)
 


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